Delineation of the recombination sites necessary for integration of pathogenicity islands II and III into the Escherichia coli 536 chromosome. 2008

Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
Universität Würzburg, Institut für Molekulare Infektionsbiologie, 97070 Würzburg, Germany.

In uropathogenic Escherichia coli strain 536, six pathogenicity islands (PAIs) encode key virulence factors. All PAIs except PAI IV(536) are flanked by direct repeats and four of them encode integrases responsible for their chromosomal excision. To study recombination sites used for the integration by PAI II(536) and III(536) integrases, we measured site-specific recombination between the chromosomal integration site attB, and the PAI-specific attachment site attP. We show that PAI III(536) IntB, but not IntA, mediates PAI III(536) integration. Studies of integrative recombination sites of both PAIs show that, when using a large cognate attP site (839 bp for PAI II(536) and 268 bp for PAI III(536)), PAI II(536) and III(536) attB sites could be reduced to 16 bp and 20 bp, respectively, without affecting recombination. Further reduction to 14 bp for PAI II(536) and 13 bp for PAI III(536) diminished recombination efficiency. Surprisingly, attP sites could also be reduced to 14 bp (PAI II(536)) and 20 bp (PAI III(536)). The integration host factor (IHF) and the DNA-bending HU protein do not influence PAI II(536) recombination, but IHF enhances PAI-III(536) excision and negatively affects its integration. These data suggest that PAI intasomes differ from those of lambda and P4 integrase paradigms.

UI MeSH Term Description Entries
D011995 Recombination, Genetic Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses. Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D002876 Chromosomes, Bacterial Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell. Bacterial Chromosome,Bacterial Chromosomes,Chromosome, Bacterial
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D044404 Genomic Islands Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands". Pathogenicity Islands,Anchored Genomic Islands,Genomic Islets,Pathogenicity Islets,Symbiosis Islands,Symbiosis Islets,Anchored Genomic Island,Genomic Island,Genomic Island, Anchored,Genomic Islands, Anchored,Genomic Islet,Island, Anchored Genomic,Island, Genomic,Island, Pathogenicity,Island, Symbiosis,Islands, Anchored Genomic,Islands, Genomic,Islands, Pathogenicity,Islands, Symbiosis,Islet, Genomic,Islet, Pathogenicity,Islet, Symbiosis,Islets, Genomic,Islets, Pathogenicity,Islets, Symbiosis,Pathogenicity Island,Pathogenicity Islet,Symbiosis Island,Symbiosis Islet
D019426 Integrases Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA. Integrase
D029968 Escherichia coli Proteins Proteins obtained from ESCHERICHIA COLI. E coli Proteins

Related Publications

Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
May 2004, Journal of bacteriology,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
November 2002, Infection and immunity,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
March 2001, The Journal of infectious diseases,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
July 2001, Infection and immunity,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
January 2009, PLoS pathogens,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
March 2000, International journal of medical microbiology : IJMM,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
October 2020, Scientific reports,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
March 1995, Journal of bacteriology,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
February 1968, Journal of molecular biology,
Caroline Wilde, and Didier Mazel, and Bianca Hochhut, and Barbara Middendorf, and Frédérique Le Roux, and Elisabeth Carniel, and Ulrich Dobrindt, and Jörg Hacker
November 1970, Japanese journal of microbiology,
Copied contents to your clipboard!