RecJ nuclease is required for SOS induction after introduction of a double-strand break in a RecA loading deficient recB mutant of Escherichia coli. 2008

Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
Department of Molecular Biology, Ruder Bosković Institute, Bijenicka 54, 10000 Zagreb, Croatia.

The SOS response is an important mechanism which allows Escherichia coli cells to maintain genome integrity. Two key proteins in SOS regulation are LexA (repressor) and RecA (coprotease). The signal for SOS induction is generated at the level of a RecA filament. Depending on the type of DNA damage, a RecA filament is produced by specific activities (helicase, nuclease and RecA loading) of either RecBCD, RecF or a hybrid recombination pathway. It was recently demonstrated that RecA loading activity is essential for the induction of the SOS response after UV-irradiation. In this paper we studied the genetic requirements for SOS induction after introduction of a double-strand break (DSB) by the I-SceI endonuclease in a RecA loading deficient recB mutant (recB1080). We monitored SOS induction by assaying beta-galactosidase activity and compared induction of the response between strains having one or more inactivated mechanisms of RecA loading and their derivatives. We found that simultaneous inactivation of both RecA loading functions (in recB1080 recO double mutant) partially impairs SOS induction after introduction of a DSB. However, we found that the RecJ nuclease is essential for SOS induction after the introduction of a DSB in the recB1080 mutant. This result indicates that RecJ is needed to prepare ssDNA for subsequent loading of RecA protein. It implies that an additional type of RecA loading could exist in the cell.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D011938 Rec A Recombinases A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure. Rec A Protein,RecA Protein,Recombinases, Rec A
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005090 Exodeoxyribonucleases A family of enzymes that catalyze the exonucleolytic cleavage of DNA. It includes members of the class EC 3.1.11 that produce 5'-phosphomonoesters as cleavage products. DNA Exonucleases,Exonucleases, DNA
D001089 Arabinose L-Arabinose,L Arabinose
D013014 SOS Response, Genetics An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive. SOS Response (Genetics),SOS Box,SOS Function,SOS Induction,SOS Region,SOS Repair,SOS Response,SOS System,Box, SOS,Function, SOS,Functions, SOS,Genetics SOS Response,Genetics SOS Responses,Induction, SOS,Inductions, SOS,Region, SOS,Regions, SOS,Repair, SOS,Repairs, SOS,Response, Genetics SOS,Response, SOS,Response, SOS (Genetics),Responses, Genetics SOS,Responses, SOS,Responses, SOS (Genetics),SOS Functions,SOS Inductions,SOS Regions,SOS Repairs,SOS Responses,SOS Responses (Genetics),SOS Responses, Genetics,SOS Systems,System, SOS,Systems, SOS
D043211 Exodeoxyribonuclease V An ATP-dependent exodeoxyribonuclease that cleaves in either the 5'- to 3'- or the 3'- to 5'-direction to yield 5'-phosphooligonucleotides. It is primarily found in BACTERIA. ATP-Dependent DNase,Exodeoxyribonuclease V, alpha Chain,Exodeoxyribonuclease V, beta Chain,Exodeoxyribonuclease V, gamma Chain,Exonuclease V,RecBC DNase,RecBC Deoxyribonuclease,RecBCD Enzyme,ATP Dependent DNase,Deoxyribonuclease, RecBC
D050296 Microbial Viability Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate. Bacterial Viability,Virus Viability,Bacteria Viability,Microbial Inactivation,Inactivation, Microbial,Viability, Bacteria,Viability, Bacterial,Viability, Microbial,Viability, Virus
D053903 DNA Breaks, Double-Stranded Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently. Double-Stranded DNA Breaks,Double-Strand DNA Breaks,Double-Stranded DNA Break,Break, Double-Strand DNA,Break, Double-Stranded DNA,Breaks, Double-Strand DNA,Breaks, Double-Stranded DNA,DNA Break, Double-Strand,DNA Break, Double-Stranded,DNA Breaks, Double Stranded,DNA Breaks, Double-Strand,Double Strand DNA Breaks,Double Stranded DNA Break,Double Stranded DNA Breaks,Double-Strand DNA Break
D029968 Escherichia coli Proteins Proteins obtained from ESCHERICHIA COLI. E coli Proteins

Related Publications

Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
February 2003, Genetics,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
February 2005, Journal of bacteriology,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
August 1980, Journal of bacteriology,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
July 2006, Journal of bacteriology,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
September 2017, DNA repair,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
February 1989, Journal of bacteriology,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
March 1995, The Journal of biological chemistry,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
May 1993, Molecular & general genetics : MGG,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
August 2009, Journal of molecular biology,
Ignacija Vlasić, and Ivana Ivancić-Baće, and Mirna Imesek, and Boris Mihaljević, and Krunoslav Brcić-Kostić
January 2000, International journal of radiation biology,
Copied contents to your clipboard!