Biomaterial Database

[Study on enzyme-assisted extraction of polysaccharides from Dioscorea opposita]. 2008

Yuan Zhang, and Qiang Lin, and Jing-Na Wei, and Hong-Ji Zhu

Biochemical Engineering College of Beijing Uion University, Beijing 100023, China.

OBJECTIVE To extract polysaccharides from Dioscorea opposita by alpha-amylase and ultrasound. METHODS The optimum condition of enzyme-assisted extraction has been obtained through orthogonal test according to the yield of polysaccharides. RESULTS A higher yield of polysaccharides was achieved at 55 degrees C, pH 5.5 , with a load of alpha-amylase 10 mg for 1.0 hour, the extraction rate was increased by in compare of sonolysis treatment alone. CONCLUSIONS To carry out an enzyme treatment before ultrasound--assisted extraction elevated the yield of polysaccharides.

UI	MeSH Term	Description	Entries
D011134	Polysaccharides	Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds.	Glycan,Glycans,Polysaccharide
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000516	alpha-Amylases	Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.	Taka-Amylase A,alpha-Amylase,Alpha-Amylase Bayer,Maxilase,Mégamylase,alpha-1,4-D-Glucanglucanohydrolase,Alpha Amylase Bayer,AlphaAmylase Bayer,Taka Amylase A,TakaAmylase A,alpha 1,4 D Glucanglucanohydrolase,alpha Amylase,alpha Amylases
D027742	Dioscorea	A plant genus best known for edible underground tubers. Yam may also refer to a moist variety of sweet potato, IPOMOEA BATATAS.	Yam,Chinese Yam,Dioscorea batatas,Dioscorea opposita,Dioscorea polystachya,Dioscorea villosa,Shan Yao,Wild Yam,Chinese Yams,Shan Yaos,Wild Yams,Yam, Chinese,Yam, Wild,Yams,Yams, Chinese,Yams, Wild,Yao, Shan,Yaos, Shan