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[Study on Deproteinization of Polysaccharides from Dioscorea nipponica]. 2016

Xiao Song, and Peng Zhao, and Xi Duan, and Chang-li Wang, and Han-shuang Deng

To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides. The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated. PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method. The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.

UI MeSH Term Description Entries
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D011506 Proteins Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein. Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D027742 Dioscorea A plant genus best known for edible underground tubers. Yam may also refer to a moist variety of sweet potato, IPOMOEA BATATAS. Yam,Chinese Yam,Dioscorea batatas,Dioscorea opposita,Dioscorea polystachya,Dioscorea villosa,Shan Yao,Wild Yam,Chinese Yams,Shan Yaos,Wild Yams,Yam, Chinese,Yam, Wild,Yams,Yams, Chinese,Yams, Wild,Yao, Shan,Yaos, Shan

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