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1. The objective of this study was to investigate the effects of four food chemicals, namely butylated hydroxytoluene (BHT), curcumin (CC), propyl gallate (PG) and thiabendazole (TB), on cytochrome P450 (CYP) forms in cultured human hepatocytes. 2. Treatment of human hepatocytes for 72 h with 2-200 microM TB produced concentration-dependent increases in CYP1A2, CYP2B6 and CYP3A4 mRNA levels, whereas treatment with BHT increased CYP2B6 and CYP3A4 mRNA levels. CYP1A2, CYP2B6 and CYP3A4 mRNA levels were induced around 48-, 21- and 9-fold, respectively, by 200 microM TB, with CYP2B6 and CYP 3A4 mRNA levels being induced around 12- and 7-fold, respectively, by 200 microM BHT. 3. In contrast, the treatment of human hepatocytes for 72 h with PG and CC had little or no effect on CYP mRNA levels. 4. The treatment of human hepatocytes with TB also induced CYP1A-dependent 7-ethoxyresorufin O-deethylase activity, whereas BHT induced CYP3A-dependent testosterone 6beta-hydroxylase activity. 5. In summary, the results demonstrate that TB is a mixed inducer of CYP forms in human hepatocytes inducing CYP1A, CYP2B and CYP3A forms, whereas BHT is an inducer of CYP2B and CYP3A forms.
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
June 1982,
Nutrition reviews,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
June 1988,
Biochemical pharmacology,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
July 1978,
Toxicology,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
March 1967,
Laboratory investigation; a journal of technical methods and pathology,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
July 1995,
Ecotoxicology and environmental safety,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
August 1994,
Biochemical and biophysical research communications,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
November 1993,
Carcinogenesis,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
August 2006,
Drug metabolism and disposition: the biological fate of chemicals,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
October 2017,
European journal of drug metabolism and pharmacokinetics,
R J Price, and
M P Scott, and
A M Giddings, and
D G Walters, and
R H Stierum, and
C Meredith, and
B G Lake
April 2003,
Drug metabolism and disposition: the biological fate of chemicals,
