Biomaterial Database

Rescue of Rous sarcoma virus (RSV) from RSV-transformed human embryonic cells by cell fusion with chick embryo fibroblasts using lysolecithin. 1976

H Ishi

Heterokaryon formation and Rous sarcoma virus (RSV)-induction were studied by fusion of RSV-transformed human embryonic cells with chick embryo fibroblasts in the presence of lysolecithin. Heterokaryon formation was observed by autoradiography. RSV-induction was identified by focus formation, electron microscopy and density gradient centrifugation of 3H-uridine-labeled particles. The most effective concentration of lysolecithin for virus induction was 10 mug/10(6) cells/0.1 ml. Efficiency of lysolecithin in virus induction was not less than that of ultraviolet-inactivated Sendai virus (UV-HVJ).

UI	MeSH Term	Description	Entries
D008244	Lysophosphatidylcholines	Derivatives of PHOSPHATIDYLCHOLINES obtained by their partial hydrolysis which removes one of the fatty acid moieties.	Lysolecithin,Lysolecithins,Lysophosphatidylcholine
D002459	Cell Fusion	Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.	Cell Fusions,Fusion, Cell,Fusions, Cell
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography
D001358	Avian Sarcoma Viruses	Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.	Avian Sarcoma Virus B77,Chicken Sarcoma Virus B77,Chicken Tumor 1 Virus,Fujinami sarcoma virus,Sarcoma Viruses, Avian,Avian Sarcoma Virus,Fujinami sarcoma viruses,Sarcoma Virus, Avian,Virus, Avian Sarcoma,Viruses, Avian Sarcoma,sarcoma virus, Fujinami,virus, Fujinami sarcoma,viruses, Fujinami sarcoma
D014776	Virus Cultivation	Process of growing viruses in live animals, plants, or cultured cells.	Viral Cultivation,Cultivation, Viral,Cultivation, Virus,Cultivations, Viral,Cultivations, Virus,Viral Cultivations,Virus Cultivations
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In