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Synthesis and biochemical studies of tetraphosphate 5' mRNA cap analogs bearing bisphosphonate modification. 2008
Anna Rydzik, and
Joanna Zuberek, and
Joanna Kowalska, and
Maciej Lukaszewicz, and
Edward Darzynkiewicz, and
Jacek Jemielity
Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.
Dinucleotide cap analogs containing tetraphosphate bridge modified by replacing one of the bridging oxygen atoms with methylene group were synthesized. The analogs have been examined for their stability towards enzymatic hydrolysis by human DcpS and binding affinity for eIF4E. Their inhibitory properties in rabbit reticulocyte lysate (RRL) translational system were studied.
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