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An RNA synthetic method with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group allows the synthesis of long oligoribonucleotides from CEM-amidites with an efficiency and final purity comparable to that obtained in DNA synthesis. The CEM-amidites give a high coupling efficiency, because the CEM group minimizes steric hindrance in the coupling reaction. The CEM group shows satisfactory stability under solid-phase synthetic conditions, avoids the generation of asymmetric centers, and is easily cleaved to give the final product. This unit describes the synthesis of the four CEM-amidites, the preparation of reagents, the solid-phase synthesis of oligoribonucleotides on an automated DNA synthesizer, and their deprotection.
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
September 1990,
Nucleic acids research,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
December 2010,
Current protocols in nucleic acid chemistry,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
February 2002,
Current protocols in nucleic acid chemistry,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
September 2012,
The Journal of organic chemistry,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
January 2000,
Nucleosides, nucleotides & nucleic acids,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
August 2011,
Journal of the American Chemical Society,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
January 2015,
Nucleosides, nucleotides & nucleic acids,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
January 1983,
Nucleic acids symposium series,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
June 1987,
Nucleic acids research,
Tadaaki Ohgi, and
Hidetoshi Kitagawa, and
Junichi Yano
August 1987,
Nucleic acids research,
