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Streamlined process for the chemical synthesis of RNA using 2'-O-thionocarbamate-protected nucleoside phosphoramidites in the solid phase. 2011
Douglas J Dellinger, and
Zoltán Timár, and
Joel Myerson, and
Agnieszka B Sierzchala, and
John Turner, and
Fernando Ferreira, and
Zoltán Kupihár, and
Geraldine Dellinger, and
Kenneth W Hill, and
James A Powell, and
Jeffrey R Sampson, and
Marvin H Caruthers
Agilent Laboratories, Agilent Technologies, Inc., 5301 Stevens Creek Boulevard, Santa Clara, California 95051, USA. doug_dellinger@agilent.com
An improved method for the chemical synthesis of RNA was developed utilizing a streamlined method for the preparation of phosphoramidite monomers and a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anhydrous conditions. The process is compatible with most standard heterobase protection and employs a 2'-O-(1,1-dioxo-1λ(6)-thiomorpholine-4-carbothioate) as a unique 2'-hydroxyl protective group. Using this approach, it was demonstrated that the chemical synthesis of RNA can be as simple and robust as the chemical synthesis of DNA.
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