Identification and characterization of the prepro-vasoactive intestinal peptide gene from the teleost Paralichthys olivaceus. 2009

Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
Biotechnology Research Institute, National Fisheries Research and Development Institute, 408-1, Sirang-ri, Gijang-eup, Gijang-gun, Busan 619-902, South Korea. bhnam@nfrdi.go.kr

The gene encoding the prepro form of a vasoactive intestinal peptide (VIP) was cloned, and its structural organization and expression profiles were determined in the teleost Paralichthys olivaceus. The prepro-VIP encodes both a VIP and a peptide-histidine-isoleucine (PHI). The VIP gene comprises six exons with two distinct peptides encoded on separate exons, i.e., PHI on exon III and VIP on exon IV. Several elements involved in cytokine-mediated activation are highly conserved in an 806-bp segment of the 5'-flanking region: cAMP responsive elements (CREs), binding sites for nuclear factor IL-6 (NF-IL-6), activating protein-1 (AP-1), stimulating protein-1 (Sp-1), two IL-6 responsive element binding proteins (IL-6 RE-BPs), and signal transducers and activators of transcription (STAT). The mRNA transcripts in normally conditioned fish are expressed in the brain, intestine, stomach, pyloric ceca, spleen, and heart, but not in the muscle, liver, head or trunk kidneys, and gill. However, prepro-VIP mRNA expression in the spleen and head kidney is significantly up- and down-regulated when exposed to an artificial bacterial challenge by Edwardsiella tarda. These results suggest that the typical features of neuropeptide VIPs are evolutionarily conserved from non-mammalian vertebrates to mammals and that the flounder VIP plays an important role in the immune system, especially inflammatory processes.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005412 Flatfishes Common name for the order Pleuronectiformes. A very distinctive group in that during development they become asymmetrical, i.e., one eye migrates to lie adjacent to the other. They swim on the eyeless side. FLOUNDER, sole, and turbot, along with several others, are included in this order. Pleuronectiformes,Scophthalmus maximus,Turbot,Psetta maxima,Solea,Turbots
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014660 Vasoactive Intestinal Peptide A highly basic, 28 amino acid neuropeptide released from intestinal mucosa. It has a wide range of biological actions affecting the cardiovascular, gastrointestinal, and respiratory systems and is neuroprotective. It binds special receptors (RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE). VIP (Vasoactive Intestinal Peptide),Vasoactive Intestinal Polypeptide,Vasointestinal Peptide,Intestinal Peptide, Vasoactive,Intestinal Polypeptide, Vasoactive,Peptide, Vasoactive Intestinal,Peptide, Vasointestinal,Polypeptide, Vasoactive Intestinal
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA

Related Publications

Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
December 2015, Marine genomics,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
February 2014, Veterinary immunology and immunopathology,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
January 2004, Marine biotechnology (New York, N.Y.),
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
October 2007, Fish & shellfish immunology,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
October 1987, The Journal of biological chemistry,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
June 2017, Fish & shellfish immunology,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
January 2011, Comparative immunology, microbiology and infectious diseases,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
March 2020, Fish & shellfish immunology,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
October 2017, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
Bo-Hye Nam, and Young-Ok Kim, and Hee Jeong Kong, and Woo-Jin Kim, and Sang-Jun Lee, and Tae-Jin Choi
August 2016, Fish physiology and biochemistry,
Copied contents to your clipboard!