BIOMAT Database Logo BIOMAT Database Logo

Disulfide-linked, gold nanoparticle based reagent for detecting small molecular weight thiols. 2009

Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Department of Chemistry & Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada.

Disulfide-linked gold nanoparticles (AuNP) were synthesized by reacting dithiobis[succinimidylpropionate] (DSP) coated nanoparticles with glutathione disulfide. AuNP-cross-linking was monitored by the red shift and broadening of the AuNP's localized surface plasmon absorption resonance (LSPR) spectrum. The exposure of the disulfide-linked AuNPs to a variety of free thiols with systematically varying molecular weight revealed a AuNP-disulfide stability to reduction by thiols up to a critical molecular weight, M(c), of >300 Da thus making the disulfide-linked AuNP the first reagent that can discriminate thiols based on their size.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D003124 Colorimetry Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004220 Disulfides Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties. Disulfide
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D013388 Succinimides A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants. Butanimides,Pyrrolidinediones
D013438 Sulfhydryl Compounds Compounds containing the -SH radical. Mercaptan,Mercapto Compounds,Sulfhydryl Compound,Thiol,Thiols,Mercaptans,Compound, Sulfhydryl,Compounds, Mercapto,Compounds, Sulfhydryl
D046529 Microscopy, Electron, Transmission Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen. Electron Diffraction Microscopy,Electron Microscopy, Transmission,Microscopy, Electron Diffraction,Transmission Electron Microscopy,Diffraction Microscopy, Electron,Microscopy, Transmission Electron
D053768 Metal Nanoparticles Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver. Metal Nanocrystals,Metallic Nanocrystals,Metallic Nanoparticles,Metal Nanocrystal,Metal Nanoparticle,Metallic Nanocrystal,Metallic Nanoparticle,Nanocrystal, Metal,Nanocrystal, Metallic,Nanocrystals, Metal,Nanocrystals, Metallic,Nanoparticle, Metal,Nanoparticle, Metallic,Nanoparticles, Metal,Nanoparticles, Metallic
D019803 Glutathione Disulfide A GLUTATHIONE dimer formed by a disulfide bond between the cysteine sulfhydryl side chains during the course of being oxidized. GSSG,Glutathione Disulfide, Ion(1-),Glutathione, Oxidized,Disulfide, Glutathione,Oxidized Glutathione

Related Publications

Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Low-molecular-weight thiols in thiol-disulfide exchange.
May 2013, Antioxidants & redox signaling,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent.
September 2008, CSH protocols,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Self-assembled gold nanoparticle molecular probes for detecting proteolytic activity in vivo.
March 2010, ACS nano,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Molecular weight-dependent genetic information transfer with disulfide-linked polyethylenimine-based nonviral vectors.
July 2013, Journal of biomaterials applications,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
A nanoparticle-based molecular beacon for directly detecting attomolar small RNA from plasma without purification.
August 2023, Talanta,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Cocaine detection using aptamer and molybdenum disulfide-gold nanoparticle-based sensors.
February 2020, Nanomedicine (London, England),
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Design of electrochemical detection of thiols based on the cleavage of the disulfide bond coupled with thionine modified gold nanoparticle-assisted amplification.
October 2013, Chemical communications (Cambridge, England),
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Disulfide-linked high molecular weight protein associated with human cataract.
July 1978, Proceedings of the National Academy of Sciences of the United States of America,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma.
October 2009, Biosensors & bioelectronics,
Suzanne Durocher, and Asad Rezaee, and Caroline Hamm, and Chitra Rangan, and Silvia Mittler, and Bulent Mutus
Detection of aldehydes by gold nanoparticle colorimetric array based on Tollens' reagent.
November 2021, Analytical methods : advancing methods and applications,
Copied contents to your clipboard!