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A method is described by which underivatized metoprolol enantiomers in plasma can be quantitated by high-performance liquid chromatography with fluorescence detection. Samples are prepared for injection using a simple solid-phase extraction procedure which is essentially 100% efficient for all analytes. The metoprolol enantiomers are resolved using a cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase and a hexane-ethanol-diethylamine mobile phase. Samples were tested for adaptability to autoinjection and found to be stable for at least 16 h after reconstitution in mobile phase. The automated method was determined to be precise and accurate for enantiomer concentrations as low as 10 ng base per ml and was successfully employed in a pharmacokinetic investigation.
V L Herring, and
T L Bastian, and
R L Lalonde
November 1992,
International journal of clinical pharmacology, therapy, and toxicology,
V L Herring, and
T L Bastian, and
R L Lalonde
September 1990,
Biomedical chromatography : BMC,
V L Herring, and
T L Bastian, and
R L Lalonde
November 1997,
Yao xue xue bao = Acta pharmaceutica Sinica,
V L Herring, and
T L Bastian, and
R L Lalonde
April 1994,
Journal of chromatography. A,
V L Herring, and
T L Bastian, and
R L Lalonde
November 2001,
Journal of chromatography. B, Biomedical sciences and applications,
V L Herring, and
T L Bastian, and
R L Lalonde
July 2003,
Analytical and bioanalytical chemistry,
V L Herring, and
T L Bastian, and
R L Lalonde
January 1982,
Journal of pharmaceutical sciences,
V L Herring, and
T L Bastian, and
R L Lalonde
May 1993,
Journal of chromatography,
V L Herring, and
T L Bastian, and
R L Lalonde
April 1996,
Journal of chromatography. B, Biomedical applications,
V L Herring, and
T L Bastian, and
R L Lalonde
January 1994,
Biomedical chromatography : BMC,
