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A rapid efficient method of separation of the thiamine pyrophosphokinase reaction products (ATP: thiamine pyrophosphotransferase) on the column packed with DEAE-Sephadex A-25 and their subsequent identification by direct spectrophotometry is suggested. Phosphorylation of some thiamine analogs substituted at the second position of the pyrimidine ring was studied. It was shown that in addition to thiamine, the enzyme transfers the pyrophosphate group to some of its derivatives. The vitamin analogs devoid of quaternary nitrogen in the thiazole cycle, do not form pyrophosphate ethers (thus being unable to act as substrates), whereas 2'-phenoxythiamine, 2'-methoxythiamine and especially 2'-phenylthiamine are phosphorylated at a greater rate than does the "true" substrate, thiamine, under similar conditions.
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
October 1978,
Biokhimiia (Moscow, Russia),
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
June 1969,
Biochimica et biophysica acta,
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
January 1962,
Archiv fur Mikrobiologie,
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
March 1978,
Biokhimiia (Moscow, Russia),
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
May 1975,
Biochemical and biophysical research communications,
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
December 1969,
Seikagaku. The Journal of Japanese Biochemical Society,
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
January 1979,
Biokhimiia (Moscow, Russia),
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
March 1979,
Biokhimiia (Moscow, Russia),
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
March 1969,
Analytical biochemistry,
A I Voskoboev, and
I P Chernikevich, and
Iu M Ostrovskii
January 1979,
Methods in enzymology,
