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A possibility of the use of new aliphatic and aminoarylic o-benzoquinone derivatives as acceptor substrates in NADH oxidation catalyzed by NAD(P)H: (acceptor)-oxidoreductase (EC 1.6.99.2) was demonstrated. The kinetic mechanism of this reaction was studied in a monosubstrate approach and the effective kinetic constants were determined. The stability of these o-benzoquinone derivatives in the oxidation-reduction reactions, including anzymic reduction and subsequent auto-oxidation of their diphenolic forms by oxygen, was substantiated using spectrophotometric techniques.
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