Biomaterial Database

Effect of the ATPase inhibitor protein IF1 on H+ translocation in the mitochondrial ATP synthase complex. 2009

Franco Zanotti, and Antonio Gnoni, and Roberto Mangiullo, and Sergio Papa

Dept of Medical Biochemistry, Biology and Physics, University of Bari, Bari, Italy.

The H(+) F(o)F(1)-ATP synthase complex of coupling membranes converts the proton-motive force into rotatory mechanical energy to drive ATP synthesis. The F(1) moiety of the complex protrudes at the inner side of the membrane, the F(o) sector spans the membrane reaching the outer side. The IF(1) component of the mitochondrial complex is a basic 10 kDa protein, which inhibits the F(o)F(1)-ATP hydrolase activity. The mitochondrial matrix pH is the critical factor for the inhibitory binding of the central segment of IF(1) (residue 42-58) to the F(1)-alpha/beta subunits. We have analyzed the effect of native purified IF(1) the IF(1)-(42-58) synthetic peptide and its mutants on proton conduction, driven by ATP hydrolysis or by [K(+)] gradients, in bovine heart inside-out submitochondrial particles and in liposome-reconstituted F(o)F(1) complex. The results show that IF(1), and in particular its central 42-58 segment, displays different inhibitory affinity for proton conduction from the F(1) to the F(o) side and in the opposite direction. Cross-linking of IF(1) to F(1)-alpha/beta subunits inhibits the ATP-driven H(+) translocation but enhances H(+) conduction in the reverse direction. These observation are discussed in terms of the rotary mechanism of the F(o)F(1) complex.

UI	MeSH Term	Description	Entries
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D011522	Protons	Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.	Hydrogen Ions,Hydrogen Ion,Ion, Hydrogen,Ions, Hydrogen,Proton
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D000097795	ATPase Inhibitory Protein	CA(2+) MG(2+)-ATPASE inhibitor found in membranes of mammalian ERYTHROCYTES.	Epsilon Subunit, F1-ATPase,F(0)F(1)-Inhibitor Protein,F1-ATPase Epsilon Subunit,Inhibitor Factor 1, ATPase,Na-K ATPase Inhibitor
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D018892	Proton-Motive Force	Energy that is generated by the transfer of protons or electrons across an energy-transducing membrane and that can be used for chemical, osmotic, or mechanical work. Proton-motive force can be generated by a variety of phenomena including the operation of an electron transport chain, illumination of a PURPLE MEMBRANE, and the hydrolysis of ATP by a proton ATPase. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p171)	Chemiosmotic Mechanisms,delta pH,pH gradient,deltapH,Chemiosmotic Mechanism,Proton Motive Force,Proton-Motive Forces,gradient, pH,pH, delta
D021122	Protein Subunits	Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.	Protomers,Protein Subunit,Protomer,Subunit, Protein,Subunits, Protein
D025261	Mitochondrial Proton-Translocating ATPases	Proton-translocating ATPases responsible for ADENOSINE TRIPHOSPHATE synthesis in the MITOCHONDRIA. They derive energy from the respiratory chain-driven reactions that develop high concentrations of protons within the intermembranous space of the mitochondria.	Electron Transport Complex V,Mitochondrial ATP Synthase,Respiratory Complex V,Mitochondrial ATP Synthases,Mitochondrial F(1)F(0) ATPase,ATP Synthase, Mitochondrial,ATP Synthases, Mitochondrial,ATPases, Mitochondrial Proton-Translocating,Mitochondrial Proton Translocating ATPases,Proton-Translocating ATPases, Mitochondrial