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Novel application for isothermal nucleic acid sequence-based amplification (NASBA). 2009

Anne Böhmer, and Verena Schildgen, and Jessica Lüsebrink, and Susanne Ziegler, and Ramona L Tillmann, and Michael Kleines, and Oliver Schildgen
Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.

Human bocavirus (HBoV), solely based on phylogenetic analyses, was classified as the second autonomous human parvovirus. Unfortunately, neither susceptible cell cultures nor animal models were described hitherto, thus complicating studies on viral genome structure and replication steps. A novel application of nucleic acid sequence-based amplification (NASBA) revealed that in all tested samples (100%) that became positive by NASBA the negative strand of the HBoV genome was packaged. Additionally, two of those samples also contained a detectable amount of positive strand (14.3%). The data confirm the assumed single-stranded negative-sense nature of HBoV-genomes that is independent of the viral subtype while showing that NASBA is useful not only for diagnosis.

UI MeSH Term Description Entries
D010322 Parvoviridae Infections Virus infections caused by the PARVOVIRIDAE. Parvovirus Infections,Infections, Parvoviridae,Infections, Parvovirus,Infection, Parvoviridae,Infection, Parvovirus,Parvoviridae Infection,Parvovirus Infection
D004277 DNA, Single-Stranded A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014771 Virion The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos. Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D052660 Bocavirus A genus in the subfamily PARVOVIRINAE comprising three species: Bovine parvovirus, Canine minute virus, and HUMAN BOCAVIRUS. Canine minute virus,Bovine parvovirus,Bocaviruses,Canine minute viruses,virus, Canine minute,viruses, Canine minute
D021161 Self-Sustained Sequence Replication An isothermal in-vitro nucleotide amplification process. The process involves the concomitant action of a RNA-DIRECTED DNA POLYMERASE, a ribonuclease (RIBONUCLEASES), and DNA-DIRECTED RNA POLYMERASES to synthesize large quantities of sequence-specific RNA and DNA molecules. Nucleic Acid Sequence-Based Amplification,NASBA,NASBA Analysis,Analyses, NASBA,Analysis, NASBA,NASBA Analyses,Nucleic Acid Sequence Based Amplification,Replication, Self-Sustained Sequence,Replications, Self-Sustained Sequence,Self Sustained Sequence Replication,Self-Sustained Sequence Replications,Sequence Replication, Self-Sustained,Sequence Replications, Self-Sustained

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