BIOMAT Database Logo BIOMAT Database Logo

Typing of Mycoplasma pneumoniae by nucleic acid sequence-based amplification, NASBA. 1996

C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Department of Microbiology, University of Antwerp UIA, Wilrijk, Belgium.

Nucleic acid sequence-based amplification, NASBA, is an isothermal amplification technique for nucleic acids and was used for typing a collection of 24 Mycoplasma pneumoniae strains. A set of primers was chosen from the 16S rRNA sequence alignment of Mycoplasma species. The nucleotide sequences of the (-)RNA amplicons were determined for M. pneumoniae strains M15/83 (type 1) and FH (type 2), and revealed a one-point difference at the 16S rRNA level between the two types. Based on this result, two type-specific probes were constructed. The probes were hybridized in solution with the amplified nucleic acids of 24 M. pneumoniae strains in an enzyme-linked gel assay (ELGA). The results obtained by NASBA-based typing are in agreement with the classification of the 24 M. pneumoniae strains into two types by other typing methods, confirming the reliability of this technique.

UI MeSH Term Description Entries
D009177 Mycoplasma pneumoniae Short filamentous organism of the genus Mycoplasma, which binds firmly to the cells of the respiratory epithelium. It is one of the etiologic agents of non-viral primary atypical pneumonia in man. Eaton Agent
D005784 Gene Amplification A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication. Amplification, Gene
D012329 RNA, Bacterial Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis. Bacterial RNA
D012336 RNA, Ribosomal, 16S Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis. 16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015336 Molecular Probe Techniques The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms. Molecular Probe Technic,Molecular Probe Technics,Molecular Probe Technique,Technic, Molecular Probe,Technics, Molecular Probe,Technique, Molecular Probe,Techniques, Molecular Probe,Probe Technic, Molecular,Probe Technics, Molecular,Probe Technique, Molecular,Probe Techniques, Molecular
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D017423 Sequence Analysis, RNA A multistage process that includes cloning, physical mapping, subcloning, sequencing, and information analysis of an RNA SEQUENCE. RNA Sequence Analysis,Sequence Determination, RNA,Analysis, RNA Sequence,Determination, RNA Sequence,Determinations, RNA Sequence,RNA Sequence Determination,RNA Sequence Determinations,RNA Sequencing,Sequence Determinations, RNA,Analyses, RNA Sequence,RNA Sequence Analyses,Sequence Analyses, RNA,Sequencing, RNA
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide

Related Publications

C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Nucleic acid sequence-based amplification (NASBA).
January 1994, Methods in molecular biology (Clifton, N.J.),
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Detection of Mycoplasma pneumoniae by real-time nucleic acid sequence-based amplification.
September 2003, Journal of clinical microbiology,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Characteristics and applications of nucleic acid sequence-based amplification (NASBA).
February 2002, Molecular biotechnology,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Novel application for isothermal nucleic acid sequence-based amplification (NASBA).
June 2009, Journal of virological methods,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Detection of Mycoplasma pneumoniae in spiked clinical samples by nucleic acid sequence-based amplification.
April 2002, Journal of clinical microbiology,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Detection of animal viruses using nucleic acid sequence-based amplification (NASBA).
January 2006, Developments in biologicals,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Nucleic acid sequence-based amplification (NASBA) for the identification of mycobacteria.
October 1993, Journal of general microbiology,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA).
May 2004, Diseases of aquatic organisms,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Pyrosequencing on templates generated by asymmetric nucleic acid sequence-based amplification (asymmetric-NASBA).
December 2011, The Analyst,
C Ovyn, and D van Strijp, and M Ieven, and D Ursi, and B van Gemen, and H Goossens
Nucleic acid sequence-based amplification (NASBA) detection of medically important Candida species.
October 1999, Journal of microbiological methods,
Copied contents to your clipboard!