Effect of lipopolysaccharide on intranasal administration of liposomal Newcastle disease virus vaccine to SPF chickens. 2009

Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
Graduate Institute of Biomedical Engineering, College of Engineering, College of Medicine, National Taiwan University, Taiwan, Republic of China.

In order to potentiate the low immunogenicity of the inactivated Newcastle disease virus immunized into chickens by mucosal route, liposomes as a drug delivery system and LPS (lipopolysaccharide) as an immuno-stimulator were evaluated. Here, we report a new nasal delivery system of inactivated Newcastle disease virus (NDV) vaccine. The intranasal vaccine was based on different lipids to form MLV (multi-lamellar vehicles) liposomes. The liposomes had combined carrier and adjuvant activities, which induced strong systemic (serum) and local (lung and nasal) humoral responses in SPF (specific-pathogen-free) chickens, and provided protective immunity. PC-Lip (phosphatidylcholine-liposome) elicited significant mucosal secretary immunoglobulin A (s-IgA) levels (p<0.05) in tracheal lavage fluid and serum IgG levels (p<0.05). In response to virulent viral challenge, birds treated with PBS (phosphate buffered saline) as control group died, whereas 80% of chickens which received PC-Lip, PC-Lip-LPS, PS-Lip (phosphatidylserine-liposome), and PS-Lip-LPS survived. HAI titers were 1:2560 in the PS-Lip-LPS group and 1:1280 in the PC-Lip, PC-Lip-LPS, and PS-Lip groups after two vaccinations. The results suggest that PC-Lip or PS-Lip might thus be suitable as a potential adjuvant for mucosal vaccination against NDV in chickens.

UI MeSH Term Description Entries
D007071 Immunoglobulin A, Secretory The principle immunoglobulin in exocrine secretions such as milk, respiratory and intestinal mucin, saliva and tears. The complete molecule (around 400 kD) is composed of two four-chain units of IMMUNOGLOBULIN A, one SECRETORY COMPONENT and one J chain (IMMUNOGLOBULIN J-CHAINS). Colostral IgA,IgA, Exocrine,IgA, Secretory,SIgA,Secretory IgA,Secretory Immunoglobulin A,Exocrine IgA,IgA, Colostral
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D009521 Newcastle Disease An acute febrile, contagious, viral disease of birds caused by an AVULAVIRUS called NEWCASTLE DISEASE VIRUS. It is characterized by respiratory and nervous symptoms in fowl and is transmissible to man causing a severe, but transient conjunctivitis. Disease, Newcastle
D009522 Newcastle disease virus The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food. Avian Paramyxovirus 1,Paramyxovirus 1, Avian
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000276 Adjuvants, Immunologic Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity. Immunoactivators,Immunoadjuvant,Immunoadjuvants,Immunologic Adjuvant,Immunopotentiator,Immunopotentiators,Immunostimulant,Immunostimulants,Adjuvant, Immunologic,Adjuvants, Immunological,Immunologic Adjuvants,Immunological Adjuvant,Adjuvant, Immunological,Immunological Adjuvants
D000281 Administration, Intranasal Delivery of medications through the nasal mucosa. Drug Administration, Intranasal,Administration, Intranasal Drug,Administration, Nasal,Intranasal Administration,Intranasal Drug Administration,Administrations, Intranasal,Administrations, Intranasal Drug,Administrations, Nasal,Drug Administrations, Intranasal,Intranasal Administrations,Intranasal Drug Administrations,Nasal Administration,Nasal Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
August 2010, Veterinary journal (London, England : 1997),
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
July 2022, Veterinary medicine and science,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
December 2008, Research in veterinary science,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
January 2024, Veterinary microbiology,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
January 1996, Research in veterinary science,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
September 2017, Vaccines,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
September 2018, Archives of virology,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
October 2023, Archives of Razi Institute,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
November 1982, Australian veterinary journal,
Li-Ping Tseng, and Chwei-Jang Chiou, and Chien-Chung Chen, and Ming-Chung Deng, and Tze-Wen Chung, and Yi-You Huang, and Der-Zen Liu
October 1951, American journal of veterinary research,
Copied contents to your clipboard!