Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula. 2009

Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
SERIDA, Servicio Regional de Investigación y Desarrollo Agroalimentario, Laboratorio de Sanidad Animal, 33299 Jove, Gijón, Spain. abalseiro@serida.org

We describe the pathology, isolation and characterisation of a virus responsible for an outbreak of a systemic haemorrhagic disease causing high mortality in tadpoles of the common midwife toad Alytes obstetricans in the 'Picos de Europa' National Park in northern Spain. The virus, provisionally designated as the common midwife toad virus (CMTV), was isolated from homogenates of visceral tissue from diseased toad tadpoles following inoculation on epithelioma papulosum cyprini (EPC) cells. Molecular characterisation of the virus, including sequence analysis of the DNA polymerase and major capsid protein genes, showed that the isolated virus was a ranavirus with marked sequence identity to other members of the genus Ranavirus. A rabbit antiserum raised against purified virions was prepared and used to definitively demonstrate systemic distribution of the virus in diseased tadpoles, indicating that the isolated virus was the primary pathogen.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D002025 Bufonidae The family of true toads belonging to the order Anura. The genera include Bufo, Ansonia, Nectophrynoides, and Atelopus. Bufo,Toads, True,Bufos,Toad, True,True Toad,True Toads
D004266 DNA Virus Infections Diseases caused by DNA VIRUSES. Infections, DNA Virus,DNA Virus Infection,Infection, DNA Virus,Virus Infection, DNA,Virus Infections, DNA
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013030 Spain Country located between France on the northeast and Portugal on the west and bordered by the Atlantic Ocean and the Mediterranean Sea. The capital is Madrid. Balearic Islands,Canary Islands
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D017995 Ranavirus A genus of IRIDOVIRIDAE which infects fish, amphibians and reptiles. It is non-pathogenic for its natural host, Rana pipiens, but is lethal for other frogs, toads, turtles and salamanders. Frog virus 3 is the type species. Frog Viruses,Frog virus 3,Frog Virus,Ranaviruses

Related Publications

Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
December 2015, Molecular phylogenetics and evolution,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
January 1983, Hearing research,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
March 1970, Oecologia,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
January 1979, Brain, behavior and evolution,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
June 2009, Peptides,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
September 2013, Ecology and evolution,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
October 1972, Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
December 2002, Die Naturwissenschaften,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
March 1968, Experientia,
Ana Balseiro, and Kevin P Dalton, and Ana del Cerro, and Isabel Marquez, and Andrew A Cunningham, and Francisco Parra, and José M Prieto, and R Casais
January 1960, Comptes rendus des seances de la Societe de biologie et de ses filiales,
Copied contents to your clipboard!