Minor modification to official method 2000.13, Reveal for E. coli O157:H7 test system in selected foods (8-hour method): validation of the test for 375 g raw beef samples. 2009

Susan Alles, and Justina Kennedy, and Alicia Rider, and Michael Wendorf, and Mark Mozola
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.

A lateral flow immunoassay for Escherichia coli O157:H7 (Reveal E. coli O157:H7, 8-h version), previously validated for 25 g raw ground beef, raw beef cubes, and lettuce rinse samples, has been validated for use in testing 375 g samples of raw ground beef and raw beef cubes. The Reveal method, including culture confirmation of positive immunoassay results, was evaluated in comparison to the current reference culture procedure of the U.S. Department of Agriculture-Food Safety and Inspection Service. For both sample types, the Reveal test with 12-h enrichment produced more positives than the reference method, although the differences were not statistically significant. Statistical equivalence to the reference method was also obtained at 10 and 11 h of enrichment for raw beef cubes and at 8, 10, and 11 h for raw ground beef, but maximum sensitivity of the method is only achieved after 12 h of enrichment. The Reveal method exhibited 100% specificity, with no false-positive results obtained on uninoculated control samples. It is recommended that this minor modification to Method 2000.13 be adopted Revised First Action.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D008460 Meat The edible portions of any animal used for food including cattle, swine, goats/sheep, poultry, fish, shellfish, and game. Meats
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014481 United States A country in NORTH AMERICA between CANADA and MEXICO.
D016885 United States Department of Agriculture A cabinet department in the Executive Branch of the United States Government concerned with improving and maintaining farm income and developing and expanding markets for agricultural products. Through inspection and grading services it safeguards and insures standards of quality in food supply and production. Department of Agriculture (U.S.),USDA,United States. Dept. of Agriculture
D018545 Lactuca Any of the various plants of the genus Lactuca, especially L. sativa, cultivated for its edible leaves. (From American Heritage Dictionary, 2d ed) Iceberg Lettuce,Lactuca sativa,Lettuce,Iceberg Lettuces,Lettuce, Iceberg,Lettuces,Lettuces, Iceberg

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