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Recent data suggest that DNA polymerases alpha and delta might have a coordinate functional role at the replication fork. In this communication we show that Mg2+ is likely the natural metal activator for both enzymes. Mn2+, a known mutagenic agent, is a competitive inhibitor of Mg2+ for DNA polymerase delta and acompetitive for DNA polymerase alpha. The 3'----5' exonuclease activity associated with DNA polymerase delta is not affected upon addition of Mn2+. Be2+, another mutagenic agent, on the other hand, has an inhibitory effect on the 3'----5' exonuclease, but not on the DNA polymerase delta. The data presented might explain the mutagenic and carcinogenic potential of these two divalent cations.
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
March 1989,
Nucleic acids research,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
November 1991,
Biochemistry,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
April 1988,
Biochemistry,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
June 1991,
The Journal of biological chemistry,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
June 1976,
Biochemistry,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
July 1987,
Proceedings of the National Academy of Sciences of the United States of America,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
February 2002,
TheScientificWorldJournal,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
January 1992,
Chromosoma,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
October 1992,
Proceedings of the National Academy of Sciences of the United States of America,
F Focher, and
A Verri, and
G Maga, and
S Spadari, and
U Hübscher
January 1981,
Progress in nucleic acid research and molecular biology,
