Oxidative damage to neurons caused by the induction of microglial NADPH oxidase in encephalomyocarditis virus infection. 2010

Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Reactive oxygen species (ROS) play an important role in diverse vital functions including host defense via anti-viral and anti-bacterial effects, but ROS also lead to peroxynitrite and hydroxyl radical production, which are powerful mediators of brain injury in brain inflammation. It is known that NADPH oxidases (NOX) are the major source of ROS. In the present study, NOX2 expression and distribution were examined after intracranial encephalomyocarditis virus B variant (EMCV-B) infection, which causes encephalitis. The reverse transcriptase (RT)-polymerase chain reaction (PCR) and immunohistochemistry showed that the expression and distribution of NOX2 were significantly up-regulated after EMCV-B infection in microglial cells, which invaded into the surrounding regions where neurons were subjected to oxidative stress. These findings suggest that the oxidative stress generated by NOX2 in activated microglial cells damages neurons and that this is an important process in the pathogenesis of EMCV-B infection.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007739 L Cells A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS. Earle's Strain L Cells,L Cell Line,L Cells (Cell Line),L-Cell Line,L-Cells,L-Cells, Cell Line,L929 Cell Line,L929 Cells,NCTC Clone 929 Cells,NCTC Clone 929 of Strain L Cells,Strain L Cells,Cell Line L-Cell,Cell Line L-Cells,Cell Line, L,Cell Line, L929,Cell Lines, L,Cell, L,Cell, L (Cell Line),Cell, L929,Cell, Strain L,Cells, L,Cells, L (Cell Line),Cells, L929,Cells, Strain L,L Cell,L Cell (Cell Line),L Cell Lines,L Cell, Strain,L Cells, Cell Line,L Cells, Strain,L-Cell,L-Cell Lines,L-Cell, Cell Line,L929 Cell,Strain L Cell
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D004686 Encephalomyocarditis virus The type species of CARDIOVIRUS causing encephalomyelitis and myocarditis in rodents, pigs, and monkeys. Infection in man has been reported with CNS involvement but without myocarditis. EMCV
D005260 Female Females
D000074662 NADPH Oxidase 2 An NADPH oxidase that is expressed by PHAGOCYTES where it transfers electrons across the plasma membrane from cytosolic NADPH to molecular oxygen on the exterior. It regulates proton (H+) flux into resting phagocytes to control intracellular pH. Mutations in the CYBB gene are associated with X-LINKED CHRONIC GRANULOMATOUS DISEASE. CYBB Protein,Chronic Granulomatous Disease Protein,Cytochrome B-245 Beta Chain,GP91-PHOX Protein,NOX2 Protein,Cytochrome B 245 Beta Chain,GP91 PHOX Protein,Oxidase 2, NADPH
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications

Related Publications

Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
April 2005, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
October 2009, Journal of immunology (Baltimore, Md. : 1950),
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
December 2008, Current aging science,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
July 2020, Neuropharmacology,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
August 2011, Journal of applied toxicology : JAT,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
April 2009, Emerging infectious diseases,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
May 2016, Journal of neuroinflammation,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
November 2006, Neurobiology of aging,
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
April 1959, Munchener medizinische Wochenschrift (1950),
Yasuhisa Ano, and Akikazu Sakudo, and Tetsuya Kimata, and Ryuta Uraki, and Katsuaki Sugiura, and Takashi Onodera
November 2006, Journal of neuroinflammation,
Copied contents to your clipboard!