The cardiac mechanical restitution was compared in papillary muscles between the active and the hibernating Richardson's ground squirrels at 0.1, 2.8, and 5 mM external Ca2+ concentration [( Ca2+]o). The amplitude of the restitution was significantly higher in hibernating animals between 37 and 7 degrees C at all [Ca2+]o. The first postrest contraction (F1) was highest at 20 degrees C and lower at 37 and 7 degrees C in both groups. The pause duration for maximum F1 was 30 s in active but 10 s in hibernating animals at 37 degrees C and increased to 100 s in both groups at 7 degrees C. The postrest potentiation was eliminated by 10(-6) M ryanodine at 20 degrees C in both groups, and this inhibitory effect was more pronounced in the hibernating group. Together, our results suggest that the activator Ca2+ for excitation-contraction coupling is mainly derived from the sarcoplasmic reticulum (SR) pool in both active and hibernating ground squirrel, and the dependence on SR Ca2+ release via ryanodine-sensitive Ca2+ channels is more marked in the hibernating state. Furthermore, there is no significant difference in sensitivity of the cardiac mechanical restitution to [Ca2+]o between the active and the hibernating condition.