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Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2.
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
February 2011,
Science (New York, N.Y.),
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
November 2010,
Science (New York, N.Y.),
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
January 2008,
Postepy biochemii,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
May 2012,
Cold Spring Harbor perspectives in biology,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
January 2014,
Annual review of biochemistry,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
December 2011,
Science (New York, N.Y.),
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
April 2002,
Cell,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
May 1996,
The Journal of cell biology,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
June 2019,
Annual review of biochemistry,
Derek J Taylor, and
Batsal Devkota, and
Andrew D Huang, and
Maya Topf, and
Eswar Narayanan, and
Andrej Sali, and
Stephen C Harvey, and
Joachim Frank
May 2024,
Annual review of biochemistry,
