-transparent.png){kind=logo}
A new protocol for testing vapors and gases in the L5178Y mouse lymphoma assay is presented. Four chemicals, propylene, 1,2-propylene oxide, 1,3-butadiene, and vinylidene chloride, were tested for their mutagenic potential. Cultures were exposed to the chemicals, which were delivered as vapors or gases, for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 microgram/ml. Each chemical was tested at least twice. Significant responses were obtained with 1,2-propylene oxide and vinylidene chloride, but neither cytotoxicity nor mutagenicity was induced by 1,3-butadiene; propylene could not be classified as either mutagenic or non-mutagenic in the assay. Rat liver S9 mix was not a requirement for the mutagenic activity of 1,2-propylene oxide, whereas the liver preparation markedly enhanced both the cytotoxicity and mutagenicity of vinylidene chloride.
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
August 1983,
Mutation research,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
January 2013,
Methods in molecular biology (Clifton, N.J.),
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
January 2019,
Methods in molecular biology (Clifton, N.J.),
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
February 1984,
Mutation research,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
May 1978,
Journal - Association of Official Analytical Chemists,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
January 1987,
Environmental mutagenesis,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
January 1994,
Environmental and molecular mutagenesis,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
January 1979,
Mutation research,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
June 1988,
Mutation research,
D McGregor, and
A G Brown, and
P Cattanach, and
I Edwards, and
D McBride, and
C Riach, and
W Shepherd, and
W J Caspary
October 1982,
Mutation research,
