BIOMAT Database Logo BIOMAT Database Logo

Modulation of extracellular matrix gene expression by heparin and endothelial cell growth factor in human smooth muscle cells. 1991

E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania.

We have previously shown that human vascular smooth muscle cells grown for several passages in the presence of heparin and endothelial cell growth factor (ECGF) exhibit profound alterations in the synthesis of extracellular matrix proteins. In the present study, we demonstrate that the mRNA steady-state levels for various matrix macromolecules were altered in the presence of heparin and ECGF, but not ECGF alone. Specifically, the expression of types I and IV collagens, fibronectin, and decorin proteoglycan genes were markedly inhibited, whereas that of versican proteoglycan and beta-actin genes were unaffected. The effects were fully reversible. The suppression of the collagen gene expression was related to decreased collagen production by cells incubated with heparin and ECGF. Thus, heparin and ECGF synergistically elicit a coordinate and selective inhibition of matrix gene expression in human smooth muscle cells.

UI MeSH Term Description Entries
D007083 Iliac Artery Either of two large arteries originating from the abdominal aorta; they supply blood to the pelvis, abdominal wall and legs. Deep Circumflex Iliac Artery,Arteries, Iliac,Artery, Iliac,Iliac Arteries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005353 Fibronectins Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins. Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D006493 Heparin A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts. Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000199 Actins Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle. F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin

Related Publications

E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Extracellular matrix gene expression by human endothelial and smooth muscle cells.
December 1991, Matrix (Stuttgart, Germany),
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1.
January 1999, In vitro cellular & developmental biology. Animal,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Heparin and endothelial cell growth factor modulate collagen and proteoglycan production in human smooth muscle cells.
August 1989, Biochemical and biophysical research communications,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Inhibition of gene expression of heparin-binding epidermal growth factor-like growth factor by extracellular superoxide dismutase in rat aortic smooth muscle cells.
June 2006, Free radical research,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Human vascular smooth muscle cells both express and respond to heparin-binding growth factor I (endothelial cell growth factor).
October 1987, Proceedings of the National Academy of Sciences of the United States of America,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Expression of stem cell factor in human aortic endothelial and smooth muscle cells.
March 1997, Atherosclerosis,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Endothelin-1 expression by vascular endothelial growth factor in human umbilical vein endothelial cells and aortic smooth muscle cells.
January 2011, Hypertension in pregnancy,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Extracellular matrix-smooth muscle phenotype modulation by macrophages.
January 1990, Annals of the New York Academy of Sciences,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Vascular cell apoptosis: cell type-specific modulation by transforming growth factor-beta1 in endothelial cells versus smooth muscle cells.
April 1999, Circulation,
E M Tan, and G R Dodge, and T Sorger, and I Kovalszky, and G A Unger, and L Yang, and E M Levine, and R V Iozzo
Corticosteroids inhibit the expression of the vascular endothelial growth factor gene in human vascular smooth muscle cells.
January 1998, European journal of pharmacology,
Copied contents to your clipboard!