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In this study, the expression of extracellular matrix genes by vascular cells from human iliac blood vessels was characterized on the mRNA steady-state level by slot blot and Northern transfer analyses, as well as by in situ hybridization. Endothelial cells were isolated from adult human iliac arteries and veins, as well as from umbilical veins; smooth muscle cells were isolated from adult human iliac arteries and inferior vena cava. The results show that confluent umbilical vein endothelial cells expressed the genes that encode types I, III, IV and VI collagens, as well as fibronectin and laminin. In contrast, the iliac endothelial cells expressed the genes for types IV and V collagens, fibronectin and laminin; mRNA transcripts for types I, III and VI collagens were not detectable. The smooth muscle cells from iliac arteries or inferior vena cava displayed gene expression for types I, III, IV, V and VI collagens, fibronectin and laminin. The results indicate major differences in gene expression for the various types of collagens by human iliac endothelial and smooth muscle cells. Furthermore, the fetal-derived umbilical endothelial cells displayed differential collagen gene expression from that of adult iliac endothelial cells.
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
April 1991,
Laboratory investigation; a journal of technical methods and pathology,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
March 2003,
Genes to cells : devoted to molecular & cellular mechanisms,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
March 2000,
American journal of respiratory cell and molecular biology,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
January 1989,
Gematologiia i transfuziologiia,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
September 2004,
American journal of physiology. Gastrointestinal and liver physiology,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
February 2017,
Pediatric research,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
October 2009,
Tissue engineering. Part A,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
August 2006,
American journal of respiratory and critical care medicine,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
October 1999,
Basic research in cardiology,
E M Tan, and
E Glassberg, and
D R Olsen, and
J P Noveral, and
G A Unger, and
J Peltonen, and
M L Chu, and
E Levine, and
S Sollberg
May 1992,
Journal of vascular surgery,
