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Comparison of corneal endothelial cell density estimated with 2 noncontact specular microscopes. 2010

Yakov Goldich, and Arie L Marcovich, and Yaniv Barkana, and Morris Hartstein, and Yair Morad, and Isaac Avni, and David Zadok
Department of Ophthalmology, Assaf Harofeh Medical Center, Zerifin, Israel. doctor.goldich@gmail.com

OBJECTIVE To assess the repeatability of endothelial cell density (ECD) measurements by the EM-935 endothelial microscope and their agreement with those of the Konan-Noncon Robo SP 6000 (Noncon Robo) specular microscope. METHODS We assessed the agreement between automated and semiautomated methods of analysis of the EM-935 and the Noncon Robo by measuring ECD in 40 eyes of 20 healthy subjects and calculating the 95% limits of agreement (LoA) and plotting Bland-Altman graphs. We then evaluated the repeatability of both the methods of the EM-935 by calculating the intraclass correlation coefficients (ICC) and coefficient of variation (CV). RESULTS The mean ECD of the 40 eyes was 2531+/-244 cells/mm2 for Noncon Robo and 2483+/-159 cells/mm2 for EM-935-automated. The difference between these 2 methods was not statistically significant. The mean ECD for EM-935-semiautomated was 2635+/-190 cells/mm2 and was statistically significantly higher than the 2 other methods (p<0.001). The 95% LoA were -435 to 339 cells/mm2 for the Noncon Robo and EM-935-automated, -230 to 438 cells/mm2 for the Noncon Robo and EM-935-semiautomated, and -347 to 43 cells/mm2 for the EM-935-automated and EM-935-semiautomated. Repeatability was better for EM-935-semiautomated method compared with automated method as expressed by ICC (95% CI) of 0.80 (0.52-0.95) vs 0.50 (0.09-0.84) and coefficient of variation of 2.43% vs 2.85%, respectively. CONCLUSIONS The EM-935 specular microscope showed better repeatability for the semiautomated mode compared with the automated mode. Although measurement agreement with the Noncon Robo was somewhat better for the semiautomated mode, agreement was only moderate for both methods. This leads us to recommend that these instruments should not be used interchangeably.

UI MeSH Term Description Entries
D008297 Male Males
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002452 Cell Count The number of CELLS of a specific kind, usually measured per unit volume or area of sample. Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D004728 Endothelium, Corneal Single layer of large flattened cells covering the surface of the cornea. Anterior Chamber Epithelium,Corneal Endothelium,Endothelium, Anterior Chamber,Epithelium, Anterior Chamber,Anterior Chamber Endothelium
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face

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