Comparison and evaluation of central corneal thickness using 2 new noncontact specular microscopes and conventional pachymetry devices. 2014

Fangjun Bao, and Qinmei Wang, and Shiming Cheng, and Giacomo Savini, and Weicong Lu, and Yifan Feng, and Ye Yu, and Jinhai Huang
*School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; †Key Laboratory of Vision Science, Ministry of Health PR China, Wenzhou, Zhejiang, China; ‡Hubei University of Medicine Affiliated Taihe Hospital, Shiyan, Hubei, China; §G. B. Bietti Eye Foundation-IRCCS, Rome, Italy; and ¶Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, China.

OBJECTIVE The aim of this study was to evaluate the repeatability of central corneal thickness (CCT) measurements in normal eyes using 2 new noncontact specular microscopes (NCSMs) EM-3000 (Tomey, Japan) and SP-02 (CSO, Italy) and to compare the results with those obtained from an SP-3000P NCSM (Topcon, Japan) and ultrasound pachymetry (USP). METHODS Seventy subjects were enrolled in a prospective study. A single experienced ophthalmologist performed tests with each of the 4 instruments. Measurements were obtained in the right eye during the same session. The testing sequence of the NCSM was randomly selected. After performing noncontact examinations, the USP was performed to derive the CCT measurements. Intraoperator repeatability was analyzed using within-subject coefficient of variation and intraclass correlation coefficients. The agreement between NCSMs or NCSM and USP was assessed with Bland-Altman plots and 95% limits of agreement (LoA). RESULTS The mean CCT values measured by SP-3000P, EM-3000, SP-02, and USP were 513.66 ± 33.14 μm, 529.12 ± 33.22 μm, 549.06 ± 40.27 μm, and 539.01 ± 35.73 μm, respectively. All coefficients of variation were <1.3%, and the intraclass correlation coefficients were >0.95. There were statistically significant differences between any 2 devices as determined by CCT measurements. The mean difference between paired comparisons was >9 μm. The 95% LoA ranges were broad, and the greatest 95% LoA was found to exist between SP-3000P and SP-02. CONCLUSIONS The new NCSMs and USP all show a high intraoperator repeatability for CCT measurements in normal eyes. However, interdevice agreement was poor and prevented the comparison of CCT measurements taken with different instruments.

UI MeSH Term Description Entries
D008297 Male Males
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D011446 Prospective Studies Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group. Prospective Study,Studies, Prospective,Study, Prospective
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face

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