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Insulin-like growth factor-1 modulates Ca2+ homeostasis and apoptosis of cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. 2010

Fengjun Zhang, and Lihong Wang, and Zhen Liu, and Ziying Xing, and Huaijing Wang, and Zhenzhong Li
Department of Anatomy, Shandong University School of Medicine, Jinan, China.

Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and a potent anti-apoptotic factor. IGF-1 plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons and prevents apoptosis in DRG neurons. Whether IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by glutamate (Glu) is still unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Intracellular Ca2+ concentration ([Ca2+]i) in isolated DRG neurons using the fluorescent Ca2+ indicator fura-3 was measured by confocal laser scanning microscope (CLSM). Procaspase-3 expression was detected by Western blot analysis. Application of 0.2 mmol/L Glu evoked an increase in [Ca2+]i, confirming the excitatory effect of Glu at this stage. The decrease of procaspase-3 expression levels after application of 0.2 mmol/L Glu suggested the apoptotic effects of Glu. These effects could be inhibited by the presence of IGF-1. In conclusion, we demonstrated that IGF-1 could modulate Ca2+ homeostasis and apoptosis of sensory DRG neurons with excitotoxicity induced by Glu. Both Ca2+ homeostasis and caspase-3 processing were implicated as the underlying neuroprotective mechanisms of IGF-1.

UI MeSH Term Description Entries
D007334 Insulin-Like Growth Factor I A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor. IGF-I,Somatomedin C,IGF-1,IGF-I-SmC,Insulin Like Growth Factor I,Insulin-Like Somatomedin Peptide I,Insulin Like Somatomedin Peptide I
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D009498 Neurotoxins Toxic substances from microorganisms, plants or animals that interfere with the functions of the nervous system. Most venoms contain neurotoxic substances. Myotoxins are included in this concept. Alpha-Neurotoxin,Excitatory Neurotoxin,Excitotoxins,Myotoxin,Myotoxins,Neurotoxin,Alpha-Neurotoxins,Excitatory Neurotoxins,Excitotoxin,Alpha Neurotoxin,Alpha Neurotoxins,Neurotoxin, Excitatory,Neurotoxins, Excitatory
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D005260 Female Females
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D005727 Ganglia, Spinal Sensory ganglia located on the dorsal spinal roots within the vertebral column. The spinal ganglion cells are pseudounipolar. The single primary branch bifurcates sending a peripheral process to carry sensory information from the periphery and a central branch which relays that information to the spinal cord or brain. Dorsal Root Ganglia,Spinal Ganglia,Dorsal Root Ganglion,Ganglion, Spinal,Ganglia, Dorsal Root,Ganglion, Dorsal Root,Spinal Ganglion
D006706 Homeostasis The processes whereby the internal environment of an organism tends to remain balanced and stable. Autoregulation
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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