Apical Cl-/HCO3- exchanger stoichiometry in the modeling of HCO3- transport by pancreatic duct epithelium. 2009

Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
Department of Human Nutrition, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Pancreatic duct cells secrete a HCO(3)(-)-rich (approximately 140 mM) fluid. Using a computer model of the pancreatic duct, Sohma, et al. have demonstrated that the activity of a Cl(-)/HCO(3)(-) exchanger with a 1: 1 stoichiometry at the apical membrane would have to be suppressed in order to achieve such a HCO(3)(-)-rich secretion. Recently the apical exchanger in pancreatic ducts has been identified as SLC26A6 and this probably mediates most of Cl(-)-dependent HCO(3)(-) secretion across the apical membrane. SLC26A6 is reported to mediate electrogenic Cl(-)/2HCO(3)(-) exchange when expressed in Xenopus oocytes. To assess the implications of this 1: 2 stoichiometry for HCO(3)(-) secretion, we have reconstructed the Sohma model using MATLAB/Simulink. To do this we have formulated an expression for the turnover rate of Cl(-)/2HCO(3)(-) exchange using network thermodynamics and we have estimated the constants from published experimental data. Preliminary data suggest that the 1: 2 stoichiometry of SLC26A6 would favor HCO(3)(-) secretion at higher concentrations.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010183 Pancreatic Ducts Ducts that collect PANCREATIC JUICE from the PANCREAS and supply it to the DUODENUM. Duct of Santorini,Duct of Wirsung,Duodenal Papilla, Minor,Wirsung's Duct,Accessory Pancreatic Duct,Accessory Pancreatic Duct of Santorini,Main Pancreatic Duct,Santorini's Duct,Accessory Pancreatic Ducts,Duct, Accessory Pancreatic,Duct, Main Pancreatic,Duct, Pancreatic,Duct, Santorini's,Duct, Wirsung's,Ducts, Pancreatic,Main Pancreatic Ducts,Minor Duodenal Papilla,Minor Duodenal Papillas,Pancreatic Duct,Pancreatic Duct, Accessory,Pancreatic Duct, Main,Pancreatic Ducts, Accessory,Papilla, Minor Duodenal,Santorini Duct,Wirsung Duct,Wirsungs Duct
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D003198 Computer Simulation Computer-based representation of physical systems and phenomena such as chemical processes. Computational Modeling,Computational Modelling,Computer Models,In silico Modeling,In silico Models,In silico Simulation,Models, Computer,Computerized Models,Computer Model,Computer Simulations,Computerized Model,In silico Model,Model, Computer,Model, Computerized,Model, In silico,Modeling, Computational,Modeling, In silico,Modelling, Computational,Simulation, Computer,Simulation, In silico,Simulations, Computer
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001639 Bicarbonates Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity. Bicarbonate,Bicarbonate Ions,Hydrogen Carbonates,Bicarbonate Ion,Carbonic Acid Ions,Hydrogen Carbonate,Carbonate, Hydrogen,Carbonates, Hydrogen,Ion, Bicarbonate,Ions, Bicarbonate,Ions, Carbonic Acid
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic

Related Publications

Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
January 2007, American journal of physiology. Gastrointestinal and liver physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
May 1993, The Journal of biological chemistry,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
March 2002, American journal of physiology. Gastrointestinal and liver physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
June 2009, American journal of physiology. Gastrointestinal and liver physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
February 2001, American journal of physiology. Renal physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
July 2010, American journal of physiology. Cell physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
July 1989, Nihon rinsho. Japanese journal of clinical medicine,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
April 2007, American journal of physiology. Gastrointestinal and liver physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
October 2012, American journal of physiology. Cell physiology,
Makoto Yamaguchi, and Hiroshi Ishiguro, and Martin Steward, and Yoshiro Sohma, and Akiko Yamamoto, and Akito Shimouchi, and Takaharu Kondo
January 1990, The American journal of physiology,
Copied contents to your clipboard!