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Cotransfer of two linked human genes, coding for the enzymes thymidine kinase (TK) and galactokinase (Gak) was demonstrated following incubation of Chinese hamster TK-deficient cells with isolated human chromosomes. The 5 colonies which were isolated all expressed a stable TK-positive phenotype. Cotransfer of the human genes coding for TK and Gak has also been observed in experiments in which isolated human chromosomes were incubated with TK-deficient human-Chinese hamster cell hybrids. These receipient hybrids had lost all human chromosomes at the time of incubation. From these experiments, four colonies were isolated, all expressing an unstable TK-positive phenotype. Using chromosome staining techniques, the presence of human chromosomes could not be demonstrated in either of the transformed clonal lines obtained with the Chinese hamster and the hybrid recipient cells. This indicates that incorporation of only the fragment of the human chromosome 17, bearing the genes for TK and Gak, has occurred in the recipient cells.
G J Wullems, and
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D Bootsma
March 1977,
Somatic cell genetics,
G J Wullems, and
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February 1978,
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G J Wullems, and
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April 1980,
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G J Wullems, and
J van der Horst, and
D Bootsma
January 1976,
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G J Wullems, and
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October 1975,
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G J Wullems, and
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June 1982,
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G J Wullems, and
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G J Wullems, and
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Cytogenetics and cell genetics,
G J Wullems, and
J van der Horst, and
D Bootsma
May 1976,
Genetics,
G J Wullems, and
J van der Horst, and
D Bootsma
May 1976,
Somatic cell genetics,
