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In this study we investigated the expression of primate galactokinase in somatic cell hybrids between a thymidine kinase-deficient mouse cell line and two different primate cell lines, one of which was derived from African green monkey kidney cells and the other from chimpanzee fibroblasts. All the African green monkey-mouse hybrid clones, selected in HAT medium, expressed monkey galactokinase activity and contained a monkey chromosome similar to a human E-group chromosome. When these clones were backselected in medium containing 5-bromodeoxyuridine, both this chromosome and the monkey galactokinase activity were lost. All the hybrid clones between mouse and chimpanzee cells, which were selected in HAT medium, contained the chimpanzee chromosome 17 and expressed chimpanzee galactokinase activity. These results indicate that the linkage relationship between galactokinase and thymidine kinase has been maintained in 3 divergent primate species--man, chimpanzee, and Old World monkey.
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
February 1978,
Proceedings of the National Academy of Sciences of the United States of America,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
September 1987,
American journal of human genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
June 1977,
Human genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
January 1977,
Cytogenetics and cell genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
May 1977,
Somatic cell genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
May 1976,
Somatic cell genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
July 1984,
American journal of human genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
April 1989,
Biochemical genetics,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
December 1972,
Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
K G Orkwiszewski, and
T A Tedesco, and
W J Mellman, and
C M Croce
January 1995,
Microbiology and immunology,
