BIOMAT Database Logo BIOMAT Database Logo

Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress. 2010

Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Department of Ophthalmology, University of South Carolina, Columbia, SC 29208, USA.

The regeneration of the 11-cis-retinyl imine chromophore of rhodopsin during the visual cycle and mechanisms that control this process are central questions in the field of vision research. The retinal pigment epithelium (RPE)-specific protein RPE65 is centrally involved in the isomerization and hydrolysis of all-trans-retinyl esters. In this study, we investigated RPE65 cleavage and potential regulatory mechanisms under oxidative stress conditions. The D407 RPE cell cultures were exposed to H(2)O(2) (100-1000 microM). Changes in the levels of RPE65 and proteins related to apoptosis were investigated using gel electrophoresis and western blotting. Mass spectrometry was used to confirm the identity of RPE65. C57BL/6J (M450) and C3HeB/FeJ (L450) mice were used for in vivo experiments. We found that a novel 45kDa truncated fragment of the RPE65 protein, designated RPE45, appears in RPE cells upon light exposure or oxidative stress. RPE45 is generated in vitro by recombinant caspases via an ubiquitination-dependent mechanism. Collectively, our results indicate that oxidative stress during the visual cycle results in cleavage of RPE65.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009928 Organ Specificity Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen. Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D005136 Eye Proteins PROTEINS derived from TISSUES of the EYE. Proteins, Eye
D005260 Female Females
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Retinal pigment epithelium 65 kDa protein (RPE65): An update.
May 2022, Progress in retinal and eye research,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Promoter analysis of RPE65, the gene encoding a 61-kDa retinal pigment epithelium-specific protein.
March 1998, Investigative ophthalmology & visual science,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Enhanced apoptosis in retinal pigment epithelium under inflammatory stimuli and oxidative stress.
November 2012, Apoptosis : an international journal on programmed cell death,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
RPE65, the major retinal pigment epithelium microsomal membrane protein, associates with phospholipid liposomes.
October 1997, Archives of biochemistry and biophysics,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Rpe65 is the retinoid isomerase in bovine retinal pigment epithelium.
August 2005, Cell,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
The gene for the retinal pigment epithelium-specific protein RPE65 is localized to human 1p31 and mouse 3.
April 1994, Genomics,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Nitric oxide leads to cytoskeletal reorganization in the retinal pigment epithelium under oxidative stress.
January 2012, Advances in bioscience and biotechnology (Print),
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Autophagy and KRT8/keratin 8 protect degeneration of retinal pigment epithelium under oxidative stress.
February 2017, Autophagy,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Lutein protects retinal pigment epithelium from cytotoxic oxidative stress.
June 2014, Cutaneous and ocular toxicology,
Hyunju Lee, and Hyewon Chung, and Hilal Arnouk, and Folami Lamoke, and Richard C Hunt, and William J M Hrushesky, and Patricia A Wood, and Sung Haeng Lee, and Wan Jin Jahng
Neuroactive steroids protect retinal pigment epithelium against oxidative stress.
August 2005, Neuroreport,
Copied contents to your clipboard!