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DNA-repair capacity, by incorporation in vitro of [3H]thymidine into DNA of isolated neuronal cells and splenic lymphocytes of rat was studied as a function of age. The incubations were carried out both in the presence and absence of hydroxyurea (HU), a known inhibitor of replicative DNA synthesis. The results indicate that neurons, unlike lymphocytes, obtained from adult and old animals offer a good model system to measure the DNA-repair process without any possible interference of DNA replicative synthesis. Further, the 'spontaneous' DNA repair by unscheduled DNA synthesis (UDS) in old neurons remained unchanged as compared to the adult level. However, the response of aging neurons, in contrast to that of young and adult neurons or of lymphocytes of any age, to a mutagenic challenge like UV light is limited. It is suggested that this lack of responsive DNA-repair against a given damage may lead to a general metabolic deterioration and senescence.
K Subrahmanyam, and
K S Rao
October 1975,
Nature,
K Subrahmanyam, and
K S Rao
March 1978,
Mutation research,
K Subrahmanyam, and
K S Rao
April 1981,
Cell biology international reports,
K Subrahmanyam, and
K S Rao
December 1977,
Journal of neurochemistry,
K Subrahmanyam, and
K S Rao
February 2000,
The Journal of toxicological sciences,
K Subrahmanyam, and
K S Rao
December 1977,
Biochimica et biophysica acta,
K Subrahmanyam, and
K S Rao
July 2001,
Neuroreport,
K Subrahmanyam, and
K S Rao
January 2002,
Journal of insect physiology,
K Subrahmanyam, and
K S Rao
October 1985,
Mechanisms of ageing and development,
K Subrahmanyam, and
K S Rao
February 1977,
Experimental eye research,
