Radioimmunoassay for protein p28 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera and breast cancer extracts. 1978

J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth

The main protein of the core of murine mammary tumor virus, with a molecular weight of 28,000 (p28), was solubilized by deoxycholate treatment of the virus and purified by Ultrogel ACA-54 filtration and hydroxyapatite chromatography. This protein was used as labeled antigen in a highly specific and reproducible radioimmunoassay. Organ extracts of uninfected C57BL mice did not contain p28, but organ extracts of infected RIII mice did contain the antigen. Despite the high content in the mammary gland, the level of p28 in the other organs was identical in male and female mice. Sera of uninfected mice and the majority of the sera of infected mice did not contain the antigen. The investigation included 338 human sera (50, normal; 157, breast cancer; 77, polycystic disease; 32, benign mastopathy; 12, fibroadenoma; 10, at risk of developing breast cancer). None contained an antigen related to p28. Eight of 24 extracts of human breast cancer gave results that appeared weakly positive, possibly as a result of proteolysis. Extracts of healthy breast tissue and the serum from the breast arterial and venous blood of corresponding patients were negative.

UI MeSH Term Description Entries
D008297 Male Males
D008324 Mammary Tumor Virus, Mouse The type species of BETARETROVIRUS commonly latent in mice. It causes mammary adenocarcinoma in a genetically susceptible strain of mice when the appropriate hormonal influences operate. Bittner Virus,Mammary Cancer Virus,Mouse mammary tumor virus,Mammary Tumor Viruses, Mouse
D008325 Mammary Neoplasms, Experimental Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS. Experimental Mammary Neoplasms,Neoplasms, Experimental Mammary,Experimental Mammary Neoplasm,Mammary Neoplasm, Experimental,Neoplasm, Experimental Mammary
D008892 Milk The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals. Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D001940 Breast In humans, one of the paired regions in the anterior portion of the THORAX. The breasts consist of the MAMMARY GLANDS, the SKIN, the MUSCLES, the ADIPOSE TISSUE, and the CONNECTIVE TISSUES. Breasts
D001943 Breast Neoplasms Tumors or cancer of the human BREAST. Breast Cancer,Breast Tumors,Cancer of Breast,Breast Carcinoma,Cancer of the Breast,Human Mammary Carcinoma,Malignant Neoplasm of Breast,Malignant Tumor of Breast,Mammary Cancer,Mammary Carcinoma, Human,Mammary Neoplasm, Human,Mammary Neoplasms, Human,Neoplasms, Breast,Tumors, Breast,Breast Carcinomas,Breast Malignant Neoplasm,Breast Malignant Neoplasms,Breast Malignant Tumor,Breast Malignant Tumors,Breast Neoplasm,Breast Tumor,Cancer, Breast,Cancer, Mammary,Cancers, Mammary,Carcinoma, Breast,Carcinoma, Human Mammary,Carcinomas, Breast,Carcinomas, Human Mammary,Human Mammary Carcinomas,Human Mammary Neoplasm,Human Mammary Neoplasms,Mammary Cancers,Mammary Carcinomas, Human,Neoplasm, Breast,Neoplasm, Human Mammary,Neoplasms, Human Mammary,Tumor, Breast
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
December 1977, Cancer research,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
April 1972, Journal of the National Cancer Institute,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
May 1972, Nature: New biology,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
June 2007, The Journal of general virology,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
November 1977, Journal of the National Cancer Institute,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
January 1980, Transactions of the Association of American Physicians,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
February 1982, Indian journal of experimental biology,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
August 1980, Cancer,
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
August 1982, Journal of immunology (Baltimore, Md. : 1950),
J C Hendrick, and C François, and C M Calberg-Bacq, and C Colin, and P Franchimont, and L Gosselin, and S Kozma, and P M Osterrieth
February 1983, Cancer,
Copied contents to your clipboard!