Biomaterial Database

Regulation of hydroxyapatite formation by gelatin and type I collagen gels. 1991

N C Blumenthal, and V Cosma, and E Gomes

Department of Bioengineering, Hospital for Joint Diseases Orthopaedic Institute, New York, NY 10003.

Calcium and phosphate were allowed to diffuse into gelatin and Type I collagen gels which were then cut into slices and analyzed for ion concentrations. Solutions of calcium and phosphate were then prepared, with ion concentrations equivalent to the highest levels in the slices, and mixed together, whereupon a rapid and copious precipitation of hydroxyapatite (HA) was observed. In contrast, HA bands were not visible in the gels until 1 to 2 1/2 days after analysis. These results indicate that Type I collagen exerts a considerable inhibitory effect on HA proliferation, probably by steric blockage of nuclei and crystal formation and growth. It thus appears that Type I collagen should be added to the list of agents that perform a regulatory role in bone mineral formation.

UI	MeSH Term	Description	Entries
D010710	Phosphates	Inorganic salts of phosphoric acid.	Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D003094	Collagen	A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).	Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D005780	Gelatin	A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.	Gelafusal
D005782	Gels	Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
D006882	Hydroxyapatites	A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)	Hydroxyapatite Derivatives,Derivatives, Hydroxyapatite