The protein network surrounding the human telomere repeat binding factors TRF1, TRF2, and POT1. 2010

Richard J Giannone, and Hayes W McDonald, and Gregory B Hurst, and Rong-Fong Shen, and Yisong Wang, and Yie Liu
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.

Telomere integrity (including telomere length and capping) is critical in overall genomic stability. Telomere repeat binding factors and their associated proteins play vital roles in telomere length regulation and end protection. In this study, we explore the protein network surrounding telomere repeat binding factors, TRF1, TRF2, and POT1 using dual-tag affinity purification in combination with multidimensional protein identification technology liquid chromatography--tandem mass spectrometry (MudPIT LC-MS/MS). After control subtraction and data filtering, we found that TRF2 and POT1 co-purified all six members of the telomere protein complex, while TRF1 identified five of six components at frequencies that lend evidence towards the currently accepted telomere architecture. Many of the known TRF1 or TRF2 interacting proteins were also identified. Moreover, putative associating partners identified for each of the three core components fell into functional categories such as DNA damage repair, ubiquitination, chromosome cohesion, chromatin modification/remodeling, DNA replication, cell cycle and transcription regulation, nucleotide metabolism, RNA processing, and nuclear transport. These putative protein-protein associations may participate in different biological processes at telomeres or, intriguingly, outside telomeres.

UI MeSH Term Description Entries
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000089804 Shelterin Complex A TELOMERE cap complex consisting of telomere-specific proteins in association with telomeric DNA such as telomeric dsDNA-sDNA junction. They are involved in the protection of chromosome ends and TELOMERASE regulation and play a role in CELLULAR SENESCENCE and ageing-related pathology. In general it consists of six mostly TELOMERE-BINDING PROTEINS (POT1, RAP1, TIN2, TPP1, TRF1, and TRF2). CST Complex,Ctc1-Stn1-Ten1 Complex,POT1-TPP1 Shelterin Complex,Telomere Cap Complex,Telomere POT1-TPP1 Complex,Telomeric Capping Complex,Telomeric Stn1-Ten1 Capping Complex,Telosome,Capping Complex, Telomeric,Complex, CST,Complex, Ctc1-Stn1-Ten1,Complex, POT1-TPP1 Shelterin,Complex, Shelterin,Complex, Telomere POT1-TPP1,Complex, Telomeric Capping,Ctc1 Stn1 Ten1 Complex,POT1 TPP1 Shelterin Complex,POT1-TPP1 Complex, Telomere,Shelterin Complex, POT1-TPP1,Telomere POT1 TPP1 Complex,Telomeric Stn1 Ten1 Capping Complex,Telosomes
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D034501 Telomere-Binding Proteins Proteins that specifically bind to TELOMERES. Proteins in this class include those that perform functions such as telomere capping, telomere maintenance and telomere stabilization. Telomere-Binding Protein,Double-Stranded Telomere-Binding Proteins,Double-Stranded Telomeric Binding Protein,Single-Stranded Telomere-Binding Protein,Single-Stranded Telomere-Binding Proteins,Telomer-Binding Protein, alpha-Subunit,Telomer-Binding Protein, beta-Subunit,Telomere End-Binding Protein (TEBP),Telomere Repeat Binding Factor,Telomere Repeat Binding Factors,Telomere Repeat Binding Proteins,Telomere-Binding Proteins, Double Stranded,Telomere-Binding Proteins, Single-Stranded,alpha-Telomere-Binding Protein,beta-Telomere-Binding Protein,Double Stranded Telomere Binding Proteins,Double Stranded Telomeric Binding Protein,Protein, Telomere-Binding,Single Stranded Telomere Binding Protein,Single Stranded Telomere Binding Proteins,Telomer Binding Protein, alpha Subunit,Telomer Binding Protein, beta Subunit,Telomere Binding Protein,Telomere Binding Proteins,Telomere Binding Proteins, Double Stranded,Telomere Binding Proteins, Single Stranded,Telomere-Binding Protein, Single-Stranded,Telomere-Binding Proteins, Double-Stranded,alpha Telomere Binding Protein,alpha-Subunit Telomer-Binding Protein,beta Telomere Binding Protein,beta-Subunit Telomer-Binding Protein
D035321 Telomeric Repeat Binding Protein 1 A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the CELL CYCLE. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues. TRF1 Protein,PIN2 Protein,TERF1 Protein,TTAGGG Repeat Binding Factor 1,PIN2 Proteins,TERF1 Proteins,TRF1 Proteins
D035341 Telomeric Repeat Binding Protein 2 A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the cell cycle. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 1 in that it contains basic N-terminal amino acid residues. TRF2 Protein,TERF2 Protein,TRBF2 Protein,Trf2 Gene Product,Gene Products, Trf2,Products, Trf2 Gene,Proteins, TERF2,Proteins, TRBF2,Proteins, TRF2,TERF2 Proteins,TRBF2 Proteins,TRF2 Proteins,Trf2 Gene Products

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