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By using extraction in the presence of Ca2+ and Triton X-100 and then in the presence of EGTA without detergent, a set of Ca2+-dependent phospholipid binding proteins has been identified in the membranes of transverse tubules (T-tubules) and sarcoplasmic reticulum (SR), isolated from rabbit skeletal muscles. Longitudinal SR, junctional SR and T-tubule membranes yielded about 9, 14 and 3.3 micrograms of EGTA-soluble proteins per 1 mg of membrane protein, respectively. In the presence of 1 mM CaCl2, 68 and 33 kDa proteins of T-tubules and junctional SR as well as 30 kDa protein of T-tubules were shown to bind to liposomes made of 1:1 w/w mixtures of (i) phosphatidylcholine and (ii) phosphatidylserine, phosphatidic acid, or phosphatidyl ethanolamine. In the presence of EGTA, the above-mentioned proteins were mostly found in the supernatants. Binding of the proteins with liposomes consisting of pure phosphatidylcholine was negligible.
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
June 1994,
Biochimica et biophysica acta,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
November 1992,
Biochemical Society transactions,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
January 1993,
Acta biochimica Polonica,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
August 1990,
Analytical biochemistry,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
January 1980,
Biochimie,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
October 1978,
Federation proceedings,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
January 1977,
Ukrains'kyi biokhimichnyi zhurnal,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
January 1980,
Bio Systems,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
July 1971,
Biochemistry,
V I Melgunov, and
N A Mamedova, and
E I Akimova, and
T A Adzhimolaev
June 1985,
Muscle & nerve,
