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Genistein, an in vitro inhibitor of topoisomerase II and tyrosine kinases, elicited an inhibition of growth and increased melanin content in five human melanoma cell lines, after six days of treatment at a concentration of 45 microM. In two lines examined more thoroughly, HO and SK-MEL-131, treatment with genistein also increased other markers of differentiation, including tyrosinase activity, reactivity with CF21 monoclonal antibody, and dendrite-like structure formation. The genistein-evoked increases in melanin content and tyrosinase activity were concentration- and time-dependent. Treatment of HO and SK-MEL-131 cells with 45 microM genistein for 24 hr or 60-600 microM genistein for only 1 hr resulted in an increase in protein-linked DNA strand breaks. Our results suggest an association between the genistein-evoked, protein-linked, DNA strand breaks and the genistein-induced differentiation of human melanoma cells.
K Kiguchi, and
A I Constantinou, and
E Huberman
January 1975,
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K Kiguchi, and
A I Constantinou, and
E Huberman
March 1973,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
K Kiguchi, and
A I Constantinou, and
E Huberman
December 1972,
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K Kiguchi, and
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December 1987,
Cancer research,
K Kiguchi, and
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December 1994,
Mutation research,
K Kiguchi, and
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K Kiguchi, and
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Radiation research,
K Kiguchi, and
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K Kiguchi, and
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Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,
K Kiguchi, and
A I Constantinou, and
E Huberman
June 1969,
Nature,
