Biomaterial Database

[Zymography identification of the matrix metalloproteinases synthesized by cultured hepatic stellate cells]. 2002

Wen-Xing Zhao, and Chong-Li Liang, and Bin Zhao

Medical Laboratory of Kunming General Hospital, Kunming 650032, China.

OBJECTIVE To establish a method for identification of the activities of matrix metalloproteinases (MMPs) synthesized by cultured hepatic stellate cells (HSC). METHODS SDS-gelatin polyacrylamide gels electrophoresis was used for the identification of MMPs synthesized by cultured HSC. RESULTS MMP-2 and MMP-9 in culture medium and HSC were sensitively identified, and MMP-2 activity was higher than MMP-9 in culture medium, the changes of MMPs in culture medium and HSC were also observed after 24h medicine treatment. CONCLUSIONS Zymography is suitable for the studies on the role of HSC in the regulation of extracellular matrix turnover.

UI	MeSH Term	Description	Entries
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D055166	Hepatic Stellate Cells	Perisinusoidal cells of the liver, located in the space of Disse between HEPATOCYTES and sinusoidal endothelial cells.	Ito Cells,Cell, Hepatic Stellate,Cells, Hepatic Stellate,Cells, Ito,Hepatic Stellate Cell,Stellate Cell, Hepatic,Stellate Cells, Hepatic
D020782	Matrix Metalloproteinases	A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.	MMP,MMPs,Matrix Metalloproteinase,Metalloproteinase, Matrix,Metalloproteinases, Matrix