Biomaterial Database

[HOE642 (Cariporide) reduces the intracellular calcium overload of isolated immature rabbit ventricular myocytes induced by anoxia/reoxygenation]. 2001

J A Yang, and X Chen, and Y F Yu

Department of Cardiovascular Surgery, PLA General Hospital, Beijing 100853, China.

OBJECTIVE To observe the effects of HOE642 (Cariporide) on intracellular free calcium in isolated immature rabbit ventricular myocytes induced by anoxia/reoxygenation, and to search the protective mechanism of the Na+/H+ exchange inhibitor on immature rabbit hearts. METHODS The ventricular cardiomyocytes isolated from six New Zealand immature rabbit hearts were divided into three groups of each heart, which were attained by mean of collagenase (type II, 0.28 mg/ml) perfusion. All cells were incubated with calcium fluorescence indicator Fluo-3/AM, and then the intracellular free calcium was measured under the laser scanning confocal microscopy. As well as the creatine phosphokinase (CK) and lactic dehydrogenase (LDH) were measured. The baseline was measured after isolation without anoxia/reoxygenation. The control group received anoxic conditions for 60 minutes and reoxygenation for 30 minutes, then measured. The experiment group received the same conditions as control group with addition of HOE642 (1 micromol/L). RESULTS After anoxia/reoxygenation, the intracellular free calcium of isolated immature rabbit ventricular myocytes and CK, LDH in control group increased significantly than baseline (P < 0.01), there were no significant difference of immature myocardial [Ca+]i and CK, LDH between experiment group and baseline (P > 0.05), and the experiment group myocardial [Ca2+]i and CK, LDH reduced significantly than control (P < 0.01). CONCLUSIONS HOE642 (Cariporide) may reduce intracellular calcium overload and the enzymes level (CK, LDH) in isolated immature rabbit ventricular myocytes induced by anoxia/reoxygenation, so the protective mechanism of HOE642 on immature rabbit heart may be by inhibition of the intracellular free calcium overload.

UI	MeSH Term	Description	Entries
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003402	Creatine Kinase	A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.	Creatine Phosphokinase,ADP Phosphocreatine Phosphotransferase,ATP Creatine Phosphotransferase,Macro-Creatine Kinase,Creatine Phosphotransferase, ATP,Kinase, Creatine,Macro Creatine Kinase,Phosphocreatine Phosphotransferase, ADP,Phosphokinase, Creatine,Phosphotransferase, ADP Phosphocreatine,Phosphotransferase, ATP Creatine
D006146	Guanidines	A family of iminourea derivatives. The parent compound has been isolated from mushrooms, corn germ, rice hulls, mussels, earthworms, and turnip juice. Derivatives may have antiviral and antifungal properties.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013450	Sulfones		Sulfone
D015687	Cell Hypoxia	A condition of decreased oxygen content at the cellular level.	Anoxia, Cellular,Cell Anoxia,Hypoxia, Cellular,Anoxia, Cell,Anoxias, Cell,Anoxias, Cellular,Cell Anoxias,Cell Hypoxias,Cellular Anoxia,Cellular Anoxias,Cellular Hypoxia,Cellular Hypoxias,Hypoxia, Cell,Hypoxias, Cell,Hypoxias, Cellular
D032383	Myocytes, Cardiac	Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).	Cardiomyocytes,Muscle Cells, Cardiac,Muscle Cells, Heart,Cardiac Muscle Cell,Cardiac Muscle Cells,Cardiac Myocyte,Cardiac Myocytes,Cardiomyocyte,Cell, Cardiac Muscle,Cell, Heart Muscle,Cells, Cardiac Muscle,Cells, Heart Muscle,Heart Muscle Cell,Heart Muscle Cells,Muscle Cell, Cardiac,Muscle Cell, Heart,Myocyte, Cardiac