Primary cultures of rat hepatocytes. 1990

J Dich, and N Grunnet
The Panum Institute, University of Copenhagen, Copenhagen, Denmark.

With the advent in 1969 of the collagenase-perfusion technique for the high-yield preparation of isolated, differentiated hepatocytes (1), easy establishment of primary cultures of hepatocytes was made possible. Since then, this experimental system has been increasingly used in many research fields.

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