CIP2A expression is associated with synovial hyperplasia and invasive function of fibroblast-like synoviocytes in rheumatoid arthritis. 2012

Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul 135-710, Republic of Korea.

Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a recently identified oncoprotein that leads to cellular proliferation in cancer cells. We aim to investigate CIP2A expression in fibroblast-like synoviocytes (FLS) and its association with the histopathological grade of synovitis and the invasive function of FLS in rheumatoid arthritis (RA). CIP2A protein expression was measured in 8 RA FLS and 8 OA FLS using Western blot analysis. CIP2A mRNA expression from 19 RA FLS and 7 OA FLS was measured using real-time PCR. Synovitis score of RA FLS-matched synovial tissues was semiquantitatively measured by two independent pathologists. An in vitro cell invasion assay was performed using RA FLS treated with CIP2A small interfering RNA (siRNA) or with control vector. Western blot analysis showed that CIP2A is more frequently overexpressed in RA FLS compared with OA FLS. CIP2A mRNA expression was higher in RA FLS compared with those in OA FLS, but did not reach statistical significance (P = 0.076). In RA, total synovitis score was strongly correlated with FLS CIP2A mRNA expression (rs = 0.849, P = 0.043). TNF-α treatment induced a robust increase in the invasive function of control FLS (P = 0.0021), but no significant effect was observed in CIP2A siRNA-treated FLS. Our data demonstrate that CIP2A expression is closely associated with the histopathological score of synovitis and invasive function of FLS in RA. These results suggest that CIP2A may play a critical role in the destructive process in RA and warrant further investigation of CIP2A as a therapeutic target.

UI MeSH Term Description Entries
D006965 Hyperplasia An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells. Hyperplasias
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D010003 Osteoarthritis A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans. Arthritis, Degenerative,Osteoarthrosis,Osteoarthrosis Deformans,Arthroses,Arthrosis,Arthritides, Degenerative,Degenerative Arthritides,Degenerative Arthritis,Osteoarthritides,Osteoarthroses
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001172 Arthritis, Rheumatoid A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated. Rheumatoid Arthritis
D001324 Autoantigens Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response. Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens
D012720 Severity of Illness Index Levels within a diagnostic group which are established by various measurement criteria applied to the seriousness of a patient's disorder. Illness Index Severities,Illness Index Severity
D013583 Synovial Membrane The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID. Synovium,Membrana Synovialis Capsulae Articularis,Membrane, Synovial,Membranes, Synovial,Synovial Membranes
D014409 Tumor Necrosis Factor-alpha Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS. Cachectin,TNF-alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,Cachectin-Tumor Necrosis Factor,TNF Superfamily, Member 2,TNFalpha,Tumor Necrosis Factor,Cachectin Tumor Necrosis Factor,Tumor Necrosis Factor alpha

Related Publications

Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
January 2013, Clinical and experimental rheumatology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
June 2020, Nature reviews. Rheumatology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
January 2011, Scandinavian journal of rheumatology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
December 2022, Current opinion in pharmacology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
November 2021, Scientific reports,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
January 2018, Clinical rheumatology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
June 2010, Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
July 2002, International journal of molecular medicine,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
November 1996, Arthritis and rheumatism,
Jaejoon Lee, and Eun-Jung Park, and Ji Won Hwang, and Ji-Min Oh, and Hyungjin Kim, and Eun-Kyung Bae, and Yoon-La Choi, and Jungho Han, and Joong Kyong Ahn, and Hoon-Suk Cha, and Eun-Mi Koh
October 2020, Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,
Copied contents to your clipboard!