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We have examined the role of oxygen radicals in ethanol-induced damage to cultured rat gastric mucosal cells. Cultured cells exposed to ethanol produced superoxide anion, as assessed by the reduction of cytochrome c, in a time-related fashion. The production of superoxide anion increased dose dependently as the concentration of ethanol increased. Cellular damage increased in a similar fashion to the production of superoxide anion. Both superoxide dismutase (SOD) and catalase diminished ethanol-induced injury dose dependently. SOD and catalase were able to maintain their enzymatic activities in the presence of 15% ethanol, respectively. Pretreatment with deferoxamine, an iron-chelating agent, decreased ethanol-induced injury dose dependently. Furthermore, dimethyl sulfoxide decreased ethanol-induced damage dose dependently. We conclude that cultured gastric mucosal cells exposed to ethanol generate oxygen radicals and that the production of oxygen radicals is closely linked with ethanol-induced damage to the cells. Hydroxyl radical, produced by the iron-catalyzed Haber-Weiss reaction, seems to be the main mediator of ethanol-induced damage to gastric mucosal cells in vitro.
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
July 1988,
Digestive diseases and sciences,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
September 1996,
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
July 1987,
The American journal of physiology,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
April 1999,
The Journal of pharmacology and experimental therapeutics,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
January 1993,
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H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
December 1982,
Toxicology letters,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
October 1989,
Gastroenterologia Japonica,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
May 1993,
Gastroenterologia Japonica,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
April 2003,
Digestive diseases and sciences,
H Mutoh, and
H Hiraishi, and
S Ota, and
K J Ivey, and
A Terano, and
T Sugimoto
July 1988,
The Journal of pharmacy and pharmacology,
