-transparent.png){kind=logo}
Most cases of nephroblastoma have high plasma levels of prorenin which is biologically inactive. Plasma prorenin levels fall to normal following nephrectomy. In order to ascertain whether renin synthesis occurs in nephroblastomas we decided to search for renin-specific mRNA using a cDNA probe and Northern blot analyses on total RNA purified from snap-frozen human tumour tissue obtained at nephrectomy. We demonstrated renin-specific mRNA in 5/11 (45 per cent) nephroblastomas. It was 1.6 Kb in length, similar to the mRNA detected in normal kidney tissue and in kidneys with renal artery stenosis. In one of the cases of nephroblastoma, in which we could detect no normal renin mRNA at 1.6 Kb, the cDNA probe hybridized with a higher molecular weight mRNA 3 Kb in length. We conclude that some nephroblastomas synthesize renin.
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
September 1996,
The Journal of pathology,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
July 1984,
Journal of clinical pathology,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
May 1991,
Journal of pediatric surgery,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
March 1987,
Journal of pediatric surgery,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
February 1990,
American journal of hypertension,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
August 1992,
British journal of anaesthesia,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
April 1991,
Archives of disease in childhood,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
June 1970,
Archives of disease in childhood,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
October 2004,
Biochemical pharmacology,
G B Lindop, and
K Duncan, and
D W Millan, and
A A Gibson, and
W J Patrick, and
B J Leckie, and
G D Birnie
September 1991,
Clinical science (London, England : 1979),
