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A continuous line (DMS-79) of human pulmonary small cell carcinoma cells was shown to secrete immunoreactive adrenocorticotropin (ACTH), lipotropin, and beta-endorphin concomitantly into the culture medium. Gel filtration of the culture medium demonstrated at least five components: high molecular weight material(s) that had ACTH, lipotropin, and beta-endorphin immunoreactivities and materials similar to ACTH, beta-lipotropin, gamma-lipotropin, and beta-endorphin in their immunoreactivities and apparent molecular weights. The same components were observed when gel filtration was carried out in 6 M guanidine-HCl, and the high molecular weight material(s) appeared to consist of more than one component, with molecular weights in the range of 15,000-40,000. Immune affinity chromatography of the high molecular weight component(s) from gel filtration with a specific anti-(1-24)ACTH serum demonstrated that the ACTH, lipotropin, and beta-endorphin immunoreactivities were possessed by the same molecule(s), suggesting that ACTH, lipotropins, and beta-endorphin were derived from a common, high molecular weight precursor.
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
April 1979,
Proceedings of the National Academy of Sciences of the United States of America,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
April 1980,
Proceedings of the National Academy of Sciences of the United States of America,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
May 1978,
The Journal of clinical endocrinology and metabolism,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
January 1979,
Annales de la nutrition et de l'alimentation,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
July 1984,
Biochimica et biophysica acta,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
November 1987,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
April 1987,
European journal of obstetrics, gynecology, and reproductive biology,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
July 1981,
Biochimica et biophysica acta,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
January 1978,
Biochemical Society transactions,
X Y Bertagna, and
W E Nicholson, and
G D Sorenson, and
O S Pettengill, and
C D Mount, and
D N Orth
December 1977,
Proceedings of the National Academy of Sciences of the United States of America,
