BIOMAT Database Logo BIOMAT Database Logo

How Trp repressor binds to its operator. 1990

D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
Institut für Genetik der Universität zu Köln, FRG.

We propose that the generally accepted model of a single Trp repressor dimer binding to a center of symmetry in the natural trp operator (Otwinowski et al., 1988) is wrong. We show here that the Trp repressor binds to a sequence whose center is located four base pairs either to the right or to the left of the central axis of symmetry that was previously identified. We show that: (i) the oligonucleotide used by Otwinowski et al. is not retarded by the Trp repressor in a mobility shift assay under conditions wherein a shorter oligonucleotide carrying our consensus sequence is retarded, (ii) that methylation protection experiments on the full natural operator sequence and the short oligonucleotide protect similar patterns and (iii) that by varying every base in the shorter oligonucleotide, we can demonstrate an optimal sequence for Trp repressor binding.

UI MeSH Term Description Entries
D008745 Methylation Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed) Methylations
D008957 Models, Genetic Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment. Genetic Models,Genetic Model,Model, Genetic
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D009875 Operator Regions, Genetic The regulatory elements of an OPERON to which activators or repressors bind thereby effecting the transcription of GENES in the operon. Operator Region,Operator Regions,Operator, Genetic,Genetic Operator,Genetic Operator Region,Genetic Operator Regions,Genetic Operators,Operator Region, Genetic,Operators, Genetic,Region, Genetic Operator,Region, Operator,Regions, Genetic Operator,Regions, Operator
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
How does trp repressor bind to its operator?
December 1991, The Journal of biological chemistry,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
Interaction of the trp repressor with trp operator DNA fragments.
January 1993, European biophysics journal : EBJ,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
A repressor heterodimer binds to a chimeric operator.
August 1988, Proceedings of the National Academy of Sciences of the United States of America,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.
November 1989, Molecular and cellular biology,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
Fluorescence quenching studies of Trp repressor-operator interaction.
November 1999, Journal of protein chemistry,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
lac Operator nucleosomes. 1. Repressor binds specifically to operator within the nucleosome core.
July 1980, Biochemistry,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
Single and double loop formation when deoR repressor binds to its natural operator sites.
August 1989, Cell,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
The CTXphi repressor RstR binds DNA cooperatively to form tetrameric repressor-operator complexes.
January 2004, The Journal of biological chemistry,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
An alkaline phosphatase protection assay to investigate trp repressor/operator interactions.
January 1991, Biochemistry,
D Staacke, and B Walter, and B Kisters-Woike, and B von Wilcken-Bergmann, and B Müller-Hill
How lac repressor binds to DNA.
June 1972, Nature,
Copied contents to your clipboard!