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A number of recombinant plasmids, containing EcoRV restriction-modification genes have been constructed. Individual genes of this system were introduced into plasmids of various incompatibility groups. Promoter regions of genes encoding methylase and restrictase have been cloned and studied. With the use of specialized vector pVE8 it was shown that the efficiency of the endonuclease gene promoter is comparable with early lambda phage promoters and produced about 70% of PL efficiency. The efficiency of the methylase gene promoter region was twice less than the efficiency of the restriction endonuclease gene promoter. Plasmid with restriction endonuclease gene promoter located downstream in relation to the additional regulatable phage lambda promoter PL has been obtained. It enabled us to construct strains 30-40 fold overproducing this enzyme under conditions of inactivation of the temperature sensitive phage repressor c1857. This construction directs the production of a high level (10%) of the total cellular soluble proteins) of the EcoRV restriction enzyme. The factors that influenced the level of enzyme synthesis under induction are discussed.
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
January 2005,
Nucleic acids research,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
June 2011,
Nucleic acids research,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
January 2003,
Biochemistry. Biokhimiia,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
May 2008,
Genetika,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
August 1985,
Indian journal of biochemistry & biophysics,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
June 1996,
Nucleic acids research,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
January 1985,
Molekuliarnaia biologiia,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
December 1987,
Nucleic acids research,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
July 1990,
Nucleic acids research,
A N Kravets, and
M V Zakharova, and
A S Solonin, and
N P Kuz'min, and
V I Taniashin, and
L I Glatman, and
A F Moroz, and
A A Baev
January 1997,
Gene,
