Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-). 2012

Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, China.

The mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-) was systematically optimized in shake flasks. The ratio of the (+) to (-) strains, the lycopene cyclase inhibitors piperidine and creatinine, the trisporic acid structural analog abscisic acid, the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) precursor leucine, and the mevalonate kinase enhancer penicillin were all identified as key factors affecting lycopene biosynthesis. With an optimal ratio of 5:1 for the (+) to (-) strains and the addition of 6 g/L creatinine on day 3, the highest lycopene production was 98.1 ± 15.5 mg/L. Based on the above result, the addition of 0.1 g/L penicillin on day 4, 150 μmol/L abscisic acid on day 3 or 0.5 g/L leucine on day 4 enhanced lycopene production to 119.7 ± 17.2, 120.6 ± 12.3 and 135.2 ± 7.0 mg/L, respectively. Finally, an integrated strategy by combining the above key factors was developed, and the highest lycopene production of 156.2 ± 15.4 mg/L was obtained, which was enhanced by 134.9% comparing with its production of 66.5 ± 3.6 mg/L before the optimization process of this work. The results obtained in this study may be useful for large-scale industrial lycopene production.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D002338 Carotenoids The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons containing 4 terpene subunits. Carotenes,Carotenoid,Tetraterpene Derivatives,Tetraterpenes,Carotene,Derivatives, Tetraterpene
D003198 Computer Simulation Computer-based representation of physical systems and phenomena such as chemical processes. Computational Modeling,Computational Modelling,Computer Models,In silico Modeling,In silico Models,In silico Simulation,Models, Computer,Computerized Models,Computer Model,Computer Simulations,Computerized Model,In silico Model,Model, Computer,Model, Computerized,Model, In silico,Modeling, Computational,Modeling, In silico,Modelling, Computational,Simulation, Computer,Simulation, In silico,Simulations, Computer
D005285 Fermentation Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID. Fermentations
D000077276 Lycopene A carotenoid and red pigment produced by tomatoes, other red fruits and vegetables, and photosynthetic algae. It is a key intermediate in the biosynthesis of other carotenoids, and has antioxidant, anti-carcinogenic, radioprotective, and anti-inflammatory properties. All-trans-Lycopene,LYC-O-MATO,Lycopene, (13-cis)-isomer,Lycopene, (7-cis,7'-cis,9-cis,9'-cis)-isomer -,Lycopene, (cis)-isomer,Pro-Lycopene,Prolycopene,All trans Lycopene,LYC O MATO,LYCOMATO,Pro Lycopene
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013149 Spiroplasma A genus of gram-negative, helical bacteria, in the family SPIROPLASMATACEAE, order Entomoplasmatales, causing disease in PLANTS. It has been isolated from TICKS; INSECTS; and PLANTS.
D018920 Coculture Techniques A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states. Cocultivation,Co-culture,Coculture,Co culture,Co-cultures,Cocultivations,Coculture Technique,Cocultures

Related Publications

Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
January 2018, Methods in molecular biology (Clifton, N.J.),
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
December 2004, Applied microbiology and biotechnology,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
May 2008, Bioresource technology,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
April 2019, Foods (Basel, Switzerland),
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
January 2014, Zeitschrift fur Naturforschung. C, Journal of biosciences,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
January 2015, Applied biochemistry and biotechnology,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
May 2012, Biotechnology letters,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
July 2003, Applied and environmental microbiology,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
March 2008, Bioresource technology,
Jin-Feng Wang, and Xiu-Ji Liu, and Rui-Sang Liu, and Hong-Mei Li, and Ya-Jie Tang
March 1962, Applied microbiology,
Copied contents to your clipboard!